金属螯合亲和膜吸附分离与纯化溶菌酶的研究(Ⅲ)混合酶和蛋清(壳)中的溶菌酶提取及纯化新工艺  

Studies on the affinity membrane for adsorption and purification of lysozyme (Ⅲ) New method of purificating lysozyme from enzyme mixture and egg white (shell)

作　　者：李静[1] 陈欢林[1] 柴红[1] 

机构地区：[1]浙江大学材料与化工学院,杭州310027

出　　处：《膜科学与技术》2001年第4期5-8,共4页Membrane Science and Technology

基　　金：国家自然科学基金资助项目 (2 96 76 0 38)

摘　　要：采用亚氨二乙酸 (IDA) -Cu2 + 亲和膜分离新工艺 ,分离纯化混合酶和蛋壳中的溶菌酶 ,实验结果表明 ,亲和膜对溶菌酶的选择吸附性能良好 ,对葡萄糖氧化酶基本不吸附 ;用亲和膜直接从蛋清 (壳 )中分离提纯溶菌酶 ,通过正交实验 ,找出了最佳操作条件 ,在此条件下提取的溶菌酶纯度大于 175 0The new technique using metal ions chelated affinity membrane to purificate lysozyme from enzyme mixture and egg white (shell) was studied. The results showed that the affinity membrane had good selectivity adsorption performance to lysozyme, but less adsorption to glucose oxidase. Also the affinity was used to extract lysozyme from egg white (shell) directly. The orthogonal test was designed to find the optimal operating conditions. Under this optimal conditions the purity of lysozyme was larger than 17?500?U/mg.

关 键 词：鸡蛋清 溶菌酶 葡萄糖氧化酶 亲和膜 纯化 金属螯合 吸附分离 

分 类 号：TQ028.8[化学工程]