辐射诱发细胞内活性氧增高与DNA氧化损伤研究  

STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN ^(60)Co γ RAY IRRADIATED BEP2D CELLS

作　　者：朱茂祥[1] 杨陟华[1] 龚诒芬[1] 陆颖[1] 曹珍山[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2001年第4期270-274,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金 (3970 0 0 30 )资助

摘　　要：用60 Coγ射线照射人支气管上皮细胞 (BEP2D)。细胞内过氧化氢 (H2 O2 )和超氧阴离子(O2 · - )分别用分子探针 2’ ,7’ -二氯荧光黄双乙酸盐 (DCFH -DA)和氢化乙锭 (HE)进行标记 ,并通过流式细胞仪测定荧光产物 2’ ,7’ -二氯荧光黄 (DCF)和溴乙锭 (EB)荧光强度进行相对定量分析。DNA氧化损伤产物 8-羟基脱氧鸟嘌呤 (OH8dG)含量用高压液相色谱结合电化学方法 (HPLC-ECD)测定。结果表明 ,60Coγ射线诱发BEP2D细胞内活性氧 (ROS)水平和OH8dG含量均显著增高 ,并有良好的剂量效应关系。进一步分析显示 ,60 Coγ射线照射诱发BEP2D细胞内ROS增高与DNA氧化损伤产物OH8dG含量呈明显正相关 。HPV-16 immortalized human bronchial epithelial cells (BEP2D) were irradiated by 60 Co gamma rays. 2',7'-dichlorofluorescein and ethidium bromide, fluorescent products of the membrane-permeable dyes 2',7'-dichloroflurescin diacetate and hydroethine, respectively, were used to monitor the intracellular production of hydrogen peroxides (H 2O 2) and superoxide anions (O 2 ·- ) respectively, by flow cytometry. 8-hydroxydeoxygunosine (OH8dG), a production of oxidative DNA damage, was examined with HPLC-ECD from extracted DNA. The results showed that the ROS productions and DNA adduct OH8dG in 60 Co gamma rays irradiated BEP2D cells increased remarkably, and revealed better dose-response correlation. Further analysis indicated the positive correlation between intracellular ROS and content of OH8dG induced by 60 Co gamma rays. Therefore, the effects of radiation on cell were involved increase of intracellular ROS and its production of oxidative DNA damage.

关 键 词：^60COΓ射线照射 人支气管上皮细胞 活性氧 DNA氧化损伤 8-羟基脱氧鸟嘌呤 肿瘤 病因学 

分 类 号：R730.231[医药卫生—肿瘤] R818[医药卫生—临床医学]