不同剂量、剂量率^(32)P照射下HeLa细胞发生凋亡及其与细胞杀伤效应的关系  

APOPTOSIS IN HeLa CELL EXPOSED TO DIFFERENT DOSE、 DOSE-RATE OF ^(32)P β-IRRADIATION AND THE CORRELATION WITH CELL-KILLING EFFECACY

作　　者：冯惠茹[1] 田嘉禾[1] 张锦明[1] 江国春[2] 

机构地区：[1]中国人民解放军总医院核医学科,北京100853 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2001年第4期311-315,共5页Journal of Radiation Research and Radiation Processing

摘　　要：研究了放射性核素3 2 P照射HeLa细胞 ,在不同剂量、剂量率条件下凋亡的发生 ,以及凋亡与杀伤效果的关系。用3 2 P放射性贴片照射细胞 ,观察照射后细胞凋亡、增殖能力变化、细胞周期再分布 ,研究不同剂量、剂量率、照射时间时细胞放射响应的特点。用荧光显微镜观察凋亡细胞形态学特点 ,并进行漂浮细胞凋亡定量分析 ;流式细胞仪凋亡定量分析及平行检测细胞周期变化 ;电镜观察凋亡亚细胞形态学特点 ;克隆形成率和细胞群体倍增大小 ,评价肿瘤细胞增殖能力。结果显示 ,在研究剂量范围内 ,HeLa细胞死亡的主要方式为凋亡 ,主要发生在照射后 4 8- 72h ,随G2期阻滞的下降 ,凋亡率逐渐增加。单次照射与分次照射的比较显示 ,前者凋亡率高于分次照射 ,与克隆形成率不一致。提示 ,在不同照射方式下 。In an attempt to elucidate some aspects of the radiobiological basis of targeted radiotherapy in oncology, the apoptosis occurred have been studied in Hela cell lines after exposing to different doses and dose-rate radiation of 32 P and the relationship between apoptosis occurred and the capacity of cell proliferation, which might be of help to the understanding of targeted radiotherapy. Asynchronous Hela cells were exposed to β radiation from 32 P absorbed in filter papers which were put closely under culture dishes of growing monolayer of Hela cells. The radiation response characteristics to different dose﹑dose-rate and radiation time were evaluated through cell-proliferation assessed by the colony-forming assay, cell cycle perturbation studied by flow cytometry and quantity analysis of apoptosis analyzed by flow cytometry and fluorescence microscopy. Morphological and flow cytometry analysis showed a delayed apoptosis. The programmed cell death approached a plateau between 48-72h post-irradiation. Electron and fluorescence microscopic studies showed the presence of morphologically apoptotic cells. Single dose radiation showed a higher apoptosis ratio than multiple low dose radiation, which didn' t correlate with clonal-forming assay, suggesting apoptosis ratio at a near time point post-irradiation is not a convincing indicator of radiation efficacy in the current experimental setting.

关 键 词：^32P 放射生物学 细胞凋亡 细胞增殖 细胞周期 磷32 β辐照 放射免疫治疗 剂量率 肿瘤细胞 杀伤效应 

分 类 号：R730.5[医药卫生—肿瘤] R730.21[医药卫生—临床医学]