聚丙烯酸载体用于青霉素酰化酶的固定  

Studies of a Polyacrylic Acid as a Carrier for the Immobilization of Penicillin Acylase

作　　者：李源勋[1] 苏新清[1] 叶庆玲[1] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002

出　　处：《功能高分子学报》2001年第4期442-444,共3页Journal of Functional Polymers

摘　　要：以反应性单体丙烯酸和交联剂二乙烯基苯 ,以石油醚为致孔剂 ,通过悬浮聚合制备固定化酶的载体 ,并用于对青霉素酰化酶的固定。研究了丙烯酸与二乙烯基苯以不同摩尔比对青霉素酰化酶固定活性的影响 ,以及悬浮聚合时水油相比例的不同所合成的载体对固定化酶性能的影响。当丙烯酸和二乙烯基苯摩尔比为 84 .2∶4时合成的载体固定青霉素酰化酶的酶活为 2 784U/g ,而水油相比为 2 .75∶1(丙烯酸和二乙烯基苯摩尔比为 84 .2∶5 )时固定青霉素酰化酶的酶活达到 2 183U/g。固定青霉素酰化酶可使青霉素转化 ,得到半合成青霉素的中间体 6-氨基青霉烷酸 ,由此可制成高效、广谱。Polyacrylic acid beads crosslinked with divinyl benzene were synthesized by suspension polymerization. This polymer is an effective carrier. Penicillin acylase was immobilized on this carrier to convert benylpenicillin to 6 aminopenicillanic acid, which may be employed in the manufacture of semisynthetic penicillins. Under the different amount of substance ratio of AA to DVB and different volume ratio of water to oil, the activities of the immobilized penicillin acylase were determined. At the optimum conditions of AA (84.2) to DVB (4) and water (2.75) to oil (1.0), there activities are 2?784?U/g and 2?183 U/g (AA∶DVB=84.2∶5) respectively.

关 键 词：聚丙烯酸树脂 聚合物载体 青霉素酰化酶 固定化酶 青霉素 制备 固定 

分 类 号：TQ465.1[化学工程—制药化工] Q814.2[生物学—生物工程]