氯化汞中毒对大鼠肾小管酶活性的影响  

EFFECT OF MERCURIC CHLORIDE TOXICOSIS ON ENZYME ACTIVITY OF RAT RENAL TUBULES

作　　者：李广君 徐丹令 刘黎明 倪之挺 党泽徐 揭冰 江家婉[2] 

机构地区：[1]上海同济大学医学院解剖学教研室,上海200311 [2]上海医科大学电镜室

出　　处：《解剖学杂志》2001年第5期450-453,454,共5页Chinese Journal of Anatomy

摘　　要：目的 :检测氯化汞中毒对肾小管上皮细胞酶化学的影响。方法 :制作氯化汞中毒大鼠模型 ,以铈盐为捕捉剂 ,分别用Ogawa法、改良Hansson法和Kobayashi法对 5′ 核苷酸酶、碳酸酐酶、及Na+-K+ATP酶进行细胞化学检测 ,并对微区元素进行X 射线能谱分析。结果 :染毒组上述三种酶活性都有所降低 ,以近曲小管为著 ,X 射线能谱图有铈峰 ,但未见汞峰。结论Objective:To examine the effect of mercuric chloride toxicosis on enzyme cytochemistry of renal tubules epithelil cells in rats.Methods:5 nucleotidase (5 Nase),carbonic anhydrase (CA) and Na +-K +-ATPase were detected on the renal tubules of mercuric chloride toxic rats with the method of Ogawa Mayahara,improved Hasson and Kobayashi respectively,cerium was used as the capture agent analyzed with X ray.Results:In the groups of toxication,the activity of 3 kinds of enzymes was all declined especially in proximal tubules of kidney.The spectra of the energy dispersive X ray spectroscope showed Ce and P peaks,but no mercurium.Conclusion:The effect of mercuric chloride toxicosis on the renal tubules epithelil cells is showed in proximal tubules in rat kidney.

关 键 词：肾小管 氯化汞中毒 酶细胞化学反应 酶活性 

分 类 号：R361.2[医药卫生—病理学]