端粒酶在子宫内膜癌组织及其外周血中的表达  

Telomerase activity in tissue of human primary endometrial carcinoma and peripheral blood.

作　　者：徐韶杰[1] 王琴[1] 史恩祥[1] 沈喜[1] 高桂卿[1] 肖颖丽[1] 孔令杰[1] 

机构地区：[1]解放军第251医院妇产科,张家口075000

出　　处：《中国优生与遗传杂志》2001年第6期27-28,39,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的 :探讨端粒酶 (telomerase)在子宫内膜组织、外周血中表达与子宫内膜癌发病的关系及其相关因素。方法 :采用聚合酶链反应 -银染色法 (PCR -A)检测端粒酶在 32例子宫内膜癌患者和 16例正常对照子宫内膜组织及外周血标本中表达情况。结果 :32例子宫内膜癌标本 ,总阳性率 94%。外周血表达阳性率 6 2 %。癌组织中端粒酶表达明显高于外周血 (P<0 .0 5 )。 16例正常子宫内膜组织标本中 ,表达总阳性率为 5 0 % (8/16 )。其外周血表达总阳性率为 2 5 % (6 /16 )。正常对照两种标本端粒酶表达未达显著水平 (P >0 .0 5 )。癌患者两种标本总阳性率均明显高于对照组 (P <0 .0 5 )。结论 :端粒酶表达与子宫内膜癌的发生有密切的关系 ,同时也受子宫内膜周期性变化的影响 ,有可能成为肿瘤的标记物。Objective:To inverstigate telomerase activity in human primary endometrial carcinoma and the relationship between telomerase expression and progression of endometrial carcinoma.Methods:Telomerase activity in the malignant tissue of endometrial carcinoma and peripheral blood specimens was detected by polymerase chain raction-agrentation(PCR-A) technique for 32patients.It was also detected.In normal endometrial tissues and peripheral blood specimens for 16 cases.Results:of 32 endometrial cancer specimens.telomerase activity positive rate was 94%.In the peripheral blood,positive rate is 62%.The telomerase expression in endometrial carcinoma tissue specimens was significantly higher than the peripheral blood specimens(P<0.05).of 16 normal endometrial tissue specimens,the telomerase expression positive rate was 50%,In their perpheral blood specimens,the positive rate was 25%,In the perpheral blood of endometrial carcinoma,The difference of telomerase activity in control group's two specimens wasn't significant(P>0.05).The positive rates of telemerase were both significantly higher in two specimens of carcinoma patients than control group's(P<0.05).Conclusion:telomerase expression might contribue to the progression of endometrial carcinoma.It is influenced by endimetrial periodic change.It might beused as a good tumor marker.

关 键 词：子宫内膜癌 外周血细胞 端粒酶 聚合酶链反应 表达 

分 类 号：R737.33[医药卫生—肿瘤]