RT-PCR/Southern杂交方法检测肝癌患者外周血AFP mRNA  

Using RT-PCR/Southern blot to detect AFP mRNA in the peripheral blood of patients with hepatocellulav carcinoma

作　　者：智慧[1] 詹俊[1] 邓庆丽[1] 黄志清 周雨迁[1] 黄志明[1] 

机构地区：[1]孙逸仙纪念医院超声科,广州510120

出　　处：《胃肠病学和肝病学杂志》2001年第4期324-327,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：基金项目 :97 3分课题资助项目 (G1 9990 541 0 5)

摘　　要：目的　寻找一种敏感的方法以检外外周血中的肝癌细胞。方法　分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RAN ,进行RT -PCR/Southern杂交检测AFPmRNA。结果　原发性肝癌患者外周血AFPmRAN的检出率为 41.4% ,其检出率与肿瘤大小以及TNM分期有关 ,对照组均为阴性。结论　肝癌患者外周血中的AFPmRNA可以作为肝癌细胞的血液微小转移的标志物。Aim Searching a sensitive method to detect malignant hepatocytes in peripheral blood.Methods Using RT PCR/Southern blot to detect AFP mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma(HCC) and 41 cases without HCC (8 cases fo cirrhosis,4 cases of acute hepatitis,15 cases of chronic hepatitis,2 cases of hepatic angioma,2 cases of non HCC hepatic tumor and 10 normal volunteers).Results In the patients with HCC,AFP mRNA was found in 12 of 29 cases(41.4%).The presence of AFP mRNA in blood seemed to be correlated with the severity of HCC patients.In the control group,AFP mRNA was not detected.Conclusions RT PCR/Southern blot is a very sensitive method to detect the AFP mRNA in peripheral blood.Its presences may be an indicator of circulating HCC cells.

关 键 词：肝细胞癌 微小转移 AFP MRNA 逆转录聚合酶链式反应 SOUTHERN杂交 

分 类 号：R735.7[医药卫生—肿瘤]