多胺浓度对pGEM体外转录影响的研究  

Polyamine Regulation on pGEM in Vitro Transcription

作　　者：姚阳[1] 吕晓娟[2] 初照成[1] 时亮[1] 于成国[1] 

机构地区：[1]中国医科大学实验技术中心,沈阳110001 [2]沈阳医学院附属中心医院

出　　处：《中国医科大学学报》2001年第6期411-413,共3页Journal of China Medical University

摘　　要：目的 :研究 3种多胺在体外转录中对RNA合成的调节 ,对 3种多胺影响转录的能力进行比较。方法 :用Run-off体外转录法 ,以EcoRⅠ酶切pGEM质粒线性DNA为模板 ,由T7RNA聚合酶催化反应。结果 :3种多胺对转录的影响 ,在低浓度时随着多胺浓度的升高促进作用增强。在高浓度时随着 3种多胺浓度增加 ,促进作用逐渐减弱。结论 :多胺影响体外转录中RNA的合成 ,存在促进其合成的最适浓度。 3种多胺对转录的促进作用各不相同 ,其促进转录的能力大小依次为精胺 ,精脒 。Objective: We studied 3 kinds of polyamine, in order to find out their effects on transcription in vitro and the difference. Methods: Run off in vitro transcription was used. We applied EcoRⅠ digested plasmid pGEM DNA as template. The reaction was catalyzed by T7 RNA polymerase. Results: In the low concentration polyamine stimulated RNA synthesis. Higher concentration resulted in a fall of transcription activity. Conclusion: Polyamine influence RNA synthesis in a dose-dependent manner when they are added to the in vitro transcription system. There is an optimum polyamine concentration for stimulating transcription in vitro. The ability of stimulating transcription in vitro is different among spermine, spermidine, and putrescine. The order of their maximal stimulation is spermine, spermidine, putrescine, from higher to lower.

关 键 词：精胺 精脒 腐胺 体外转录 

分 类 号：R394.8[医药卫生—医学遗传学] R329.26[医药卫生—基础医学]