幽门螺杆菌空泡毒素基因的PCR分型与上消化道疾病的关系  

Relationship of Hp VacA Gene PCR Pattern to Gastroduodenal Disorders

作　　者：方莹[1] 王天叫[1] 张长弓[1] 颜江华[1] 李东辉[1] 

机构地区：[1]厦门大学抗癌研究中心,厦门362005

出　　处：《福建医科大学学报》2001年第3期230-231,236,共3页Journal of Fujian Medical University

摘　　要：目的　探讨幽门螺杆菌 (Hp)空泡毒素基因的两种亚型 vac A1 和 vac A2 与上消化道疾病的关系。　方法　采用特异性引物扩增 Hp尿素酶基因及 vac A基因。　结果　 6 6 .4% (81/ 12 2 )的患者胃粘膜检测出 Hp尿素酶基因 ,其中消化性溃疡患者的检出率 (74.6 % 5 3/ 71)高于慢性胃炎患者 (5 4.9% 2 8/ 5 1) ,二者差异有显著性。 vac A的两种亚型 vac A1 和 vac A2 各占 80 .2 % (6 5 / 81)和 19.8% (16 / 81) ,每种胃粘膜只能扩增出一种产物 ,其中 80 .7%(4 7/ 5 3)的消化性溃疡患者感染的是 vac A1 基因型 Hp,而慢性胃炎患者的检出率是 6 4.3% (18/ 2 8) ,差异有显著性。　结论　 vac A1 基因型 Hp与消化性溃疡关系密切。Objective\ To study two sub\|genotypes of vacA (vacA\-1 & vacA\-2) gene of Helicobacter pylori(Hp) and their relation with disease.\ Methods\ PCR was used to determine urease gene and vacA gene in Hp.\ Results\ Urease gene was found in 81(66 4%) of 122 case of Helicobacter pylori(Hp) infected patients.\ 74 6%(53/71) patients with peptic ulcer contained urease gene, which was significantly higher than those with chronic gastritis(54 9%,28/51).\ Only one sub\|genotype of vacA was detected in each specimen.\ In 81 urease gene positive cases, the positive rate of vacA\-1 gene was 80 2%(65/81), and that of vacA\-2\|gene was 19 8%(16/81).\ In 65 vacA\-1 positive cases, it was found in 80.7%(47/53) of peptic ulcer patients, and in 64.3%(18/28) of chronic gastritis.\ χ\+2 test showed that the positive rate of Hp with vacA\-1 gene in peptic ulcer differed significantly from that in chronic gastritis.\ Conclusion\ Hp with vacA\-1 gene is strongly related to peptic ulcer.\;

关 键 词：幽门螺杆菌 基因 毒素类 空泡 消化系统疾病 

分 类 号：R573[医药卫生—消化系统]