发育、成年及老年期大鼠脑中Cdk5,p35,p39的表达及Cdk5激酶活性变化  

The Expression of Cdk5, p35, p39 and Cdk5 Kinase Activity in Developing, Adult and Aged Rat Brains

作　　者：彭芳芳[1] 张百芳[1] 章江洲[1] 胡咏兵[2] 武栋成[1] 

机构地区：[1]武汉大学医学院生物化学教研室,430070 [2]武汉大学人民医院神经内科

出　　处：《卒中与神经疾病》2001年第6期350-353,共4页Stroke and Nervous Diseases

摘　　要：目的　研究不同发育时期大鼠脑中Cdk5 ,p35 ,p39的表达及Cdk5激酶活性变化。 方法　免疫印迹测定胚胎 12天至出生后 18个月大鼠脑中Cdk5 ,p35 ,p39的表达 ,通过测定Cdk5特定底物 组蛋白H1被磷酸化的程度检测鼠脑中Cdk5激酶活性。结果　从胚胎 12天至出生 7天 ,大鼠脑中Cdk5蛋白水平逐渐增加到峰值并保持至 18个月龄。在胚胎 12天时 ,Cdk5激酶活性和p35的蛋白水平较低 ,在胚胎 18天至出生 14天期间最高 ,然后在 3个月龄至 18个月龄的成年鼠和老年鼠脑中逐渐减少。与之相比 ,p39的表达方式与Cdk5和p35正好相反。自P14至 18M龄鼠脑中 ,大脑皮质及海马的 p35表达和Cdk5激酶活性高于小脑或纹状体。结论　大鼠发育早期 ,Cdk5激酶复合物的活化与神经发育密切相关 ,其生理作用在大脑皮质及海马有区域特异性。Objective To study the expression of Cdk5, p35, p39 and Cdk5 kinase activity in different developing rat brains.Methods The expression of Cdk5, p35, p39 was investigated in rat brain from embryonic day 12(E12) to postnatal 18 months (18M) by immunoblotting.An in vitro Cdk5 associated histone H 1 kinase activity (Cdk5 kinase activity) assay was carried out as described by Zheng et al.Results The Cdk5 protein levels increased from E12 to postnatal day 7 (P7) and remained at this level until 18M.The Cdk5 kinase activity and the level of p35 protein was low at E12, became prominent at E18 P14 but then decreased in the adult and aged rat brains of 3M to 18M.In comparison, the expression pattern of p39 appeared to have an inverse relationship to that of Cdk5 and p35.Conclusion The activity Cdk5 kinase complex is available early in brain development and that it is essential for neurogenesis, and which may play physiological roles related to the region specific function of the cerebral cortex and hippocampus.

关 键 词：动物实验 神经发育 细胞周期依赖性蛋白激酶 激酶活性 

分 类 号：Q55[生物学—生物化学] R-332[医药卫生]