人体良、恶性病变p53、C-erbB-2、PCNA的异常表达免疫组化检测研究  

Immunohistochemical study on the alterated expression of p53、C-erbB-2、and PCNA in humen benign and malignant lesions

作　　者：黎音[1] 卢晓梅[2] 赵春芳[2] 柳菊[3] 李同英[4] 张巍[5] 陈朝伦[1] 

机构地区：[1]新疆医科大学基础医学院病理解剖学教研室,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院包虫病实验室,新疆乌鲁木齐830054 [3]新疆医科大学附属肿瘤医院病理科,新疆乌鲁木齐830054 [4]新疆医科大学第一附属医院耳鼻喉科,新疆乌鲁木齐830054 [5]新疆医科大学第一附属医院病理科,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2001年第4期313-315,共3页Journal of Xinjiang Medical University

摘　　要：目的 :检测 p5 3、C- erb B- 2与 PCNA的异常表达可能为区别不同组织良、恶性病变提供可靠客观的指标。 方法 :对 6 6 3例恶性肿瘤 (包括鳞癌、腺癌、黑色素瘤 )及 5 6 6例良性病变 (包括乳头状瘤、腺瘤及慢性炎病变 )组织 p5 3、C- erb B- 2及 PCNA表达进行免疫组化检测。结果:恶性肿瘤 p5 3、C- erb B- 2及 PCNA表达阳性的检出率分别为 6 2 .7%、85 .8%及 94.7% ,明显较良性肿瘤与炎性病变为高 (分别为 0～ 11% ,12 .8%～ 45 .5 %及 13.6 %～6 1.8% ) (P <0 .0 5～ 0 .0 0 1)。结论:抑癌基因 p5 3的突变、癌基因 C- erb B- 2的过度表达与扩增及 PCNA的过高表达是恶性病变的共同特征 ,可以作为诊断或区别诊断良恶性病变的重要分子标志。Objective: Detection of p53?C-erbB-2?PCNA alterated expression may offer important index for diagnosis of benign and malignant human lesions. Methods: p53?C-erbB-2 and PCNA exprssions in 663 malignant (squmeous cell carcinoma, adenocarcinoma, melanoma) and 566 benign (adenoma?papilloma and chronic inflammatory) lesions were detected by immunohistochemistry (LSAB). Results: Overexpression of p53?C-erbB-2 and PCNA in malignant lesions was 62.7%, 85.8%, and 94.7% revealing significantly higher than in benign lesions (0~11%, 12.8~45.5%, and 13.6~61.8%) (P<0.05~0.001) respectively. Conclusion: Mutation of tumor suppressor gene p53, overexpression and amplification of oncogenes in human malignant lesions may be an important biomolecular marker for diagnosis or differential dignosis on both benign and malignant lesions.

关 键 词：P53 C-ERBB-2 PCNA 免疫组化 癌基因 肿瘤 

分 类 号：R730.2[医药卫生—肿瘤]