肠MALT淋巴瘤细胞凋亡与p53和bc-l2的表达  

Correlation of apoptosis with the expression of p53 and bcl-2 in primary intestinal mucosa-associated lymphoid tissue (MALT) lymphoma

作　　者：仲召阳[1] 曾盛[2] 王东[1] 肖华亮[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所病理科,重庆400042 [2]北京军区总医院病理科

出　　处：《诊断病理学杂志》2001年第5期293-295,共3页Chinese Journal of Diagnostic Pathology

摘　　要：目的　探讨肠黏膜相关淋巴组织 (MALT)淋巴瘤细胞增殖和凋亡的特征以及凋亡相关调控基因的表达。方法　应用TUNEL技术检测 2 1例肠淋巴瘤的凋亡指数 (apoptosisindex,AI) ,免疫组化S P法检测PCNA增殖指数(proliferativeindex ,PI)及bcl 2和p5 3蛋白的表达。结果　随着肠MALT淋巴瘤恶性度的增高 ,AI和PI显著增加 ,并且二者呈显著正相关。低度恶性、高度恶性伴低度恶性以及高度恶性肿瘤组中 ,bcl 2阳性率分别为 79 4%、5 7 1%、40 9%。三组bcl 2阳性率及AI均差异显著 (P <0 0 5 )。bcl 2与AI呈显著负相关 (P <0 0 5 )。p5 3阳性率为 31 4%。高度恶性组p5 3阳性率显著高于其余两组。p5 3与bcl 2表达呈负相关 (P <0 0 5 )。结论　凋亡和增殖在肿瘤的发生、发展、转化中起着重要作用 ,检测AI、PI可能是诊断恶性淋巴瘤、评价其生物学行为的可靠指标。在MALT的恶性度从低到高的转化中 ,p5 3和bclObjective To investigate the characteristics of apoptosis and proliferation and the expression of apoptosis associated antigens in intestinal MALT lymphoma. Methods Terminal dUTP nick end labeling (TUNEL) technique was used to detect apoptotic cells, and immunohistochemical staining to show the expression of p53, bcl-2 and proliferating cell nuclear antigen (PCNA) in 70 cases of intestinal MALT lymphomas. Results Apoptotic index (AI) and proliferative index (PI) increased significantly as the histological grade advanced. Positive correlation of AI with PI (r=0.8058, p<0.05) was found. Bcl-2 was expressed in 79.4% of low grade, 57.1% moderate grade and 40.0% high grade tumors. An inverse correlation was observed between the expression of bcl-2 and AI (p<0.05). Expression of p53 was found in 31.4% of intestinal MALT lymphomas. Positive rate of p53 protein was significantly increased as the histological grade of the tumors advanced (p<0.05). There was also an inverse correlation between the expression of p53 and bcl-2 proteins in the MALT lymphoma. Conclusions Apoptosis and proliferation may be important in development and progression of the tumor. Detection of AI and PI provides reliable data for the diagnosis of intestinal MALT lymphoma and for evaluation of its biological behaviors. P53 and bcl-2 are important apoptosis regulating genes that play an important role in the transformation from low grade to high grade lymphomas.

关 键 词：MALT淋巴瘤 P53 BCL-2 增殖细胞核抗原 细胞凋亡 

分 类 号：R733.4[医药卫生—肿瘤]