兔感染人巨细胞病毒AD169的初步探讨  

A preliminary study on infection of rabbits by human cytomegalovirus AD169 strain

作　　者：孟红[1] 孙广莲[1] 李焱[1] 刘菊华[1] 王玉梅[1] 

机构地区：[1]山东省医学科学院基础医学研究所微生物学研究室,济南250062

出　　处：《中华实验和临床病毒学杂志》2001年第4期374-376,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省自然基金资助 (Y96C 160 48)

摘　　要：目的　探讨兔对人巨细胞病毒 (Humancytomegalovirus,HCMV)AD16 9毒株 (静脉途径接种 )的敏感性。方法　将HCMVAD16 9接种于体外培养的兔胚肺 (Rabbitembryolung ,REL)细胞 ,观察病变 ,免疫组化检测病毒抗原 ,电镜观查细胞内病毒颗粒。 30只新西兰兔分别静脉途径接种 1万个TCID5 0、10 0个TCID5 0、灭活HCMVAD16 9,观察动物发病情况、组织病理学变化和病毒DNA。结果　REL细胞在接种病毒后 16h与HEL细胞同步开始出现细胞病变 ,免疫组化在接种病毒 16h的细胞内查到HCMV特异抗原 ,在接种病毒 36h的细胞核、浆内查到病毒颗粒。动物在接种病毒后 7d出现病态表现 ,病情与接种量呈正相关。尸体解剖发现受累器官广泛 ,在多种组织细胞内可以查到HCMV基因。结论　HCMVAD16 9株在体外REL细胞中进行病毒复制。静脉途径接种HCMVAD16 9株可以感染家兔 。Objective To study the susceptibility of rabbit to human cytomeglavirus (HCMV) in vivo and in vitro. Methods Rabbit embryo lung (REL) cell monolayer were inoculated with HCMV AD169 strain.The cytopathic effect (CPE) was observed by microscope.HCMV antigen was detected by immunohistochemical method and viral particlers were shown by electron microscopy.Human embryo lung (HEL) was taken control.Thirty New Zealand rabbits were divided into three groups randomly,two were virus groups (group1,group2) and one was inactivated virus group(group3).50 days later,the rabbits tissue lesions were observed by pathological techniques,HCMV DNA was analyzed by in situ hybridization.Results The CPE appeared 16 h post inoculation in REL cells as well as in HEL cells,at the same time,the HCMV antigen also was detected by immunhistochemistry and a large number of viral particles were manifested in the nucleus and cytoplasm of REL cells by electron microscope 36 h after virus inoculation.The extensive pathological damages in tissues of HCMV infected rabbits were observed and the viral DNA was also demonstrated in many tissues of HCMV infected rabbits.Conclusion HCMV can infect REL cells and fulfil its replication cycle in vitro. HCMV AD169 strain can infect rabbits through intravenous injection and different infection models can be established by controlling the dosage of inoculated virus.

关 键 词：疱疹病毒科 感染 家兔 动物模型 

分 类 号：R363[医药卫生—病理学]