bFGF复合NNB无机骨修复兔下颌骨缺损的实验研究  

Experimental Research of NNB Composite in Repairing Mandibular Defects in Rabbits

作　　者：蒋欣泉[1] 潘可风[2] 刘世勋 马贵骧[4] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔颌面外科,上海200011 [2]同济大学口腔医学院,上海200071 [3]上海市口腔病防治院,上海200031 [4]北京积水潭医院骨科研究所,北京100035

出　　处：《上海口腔医学》2001年第3期233-235,共3页Shanghai Journal of Stomatology

摘　　要：目的　研究碱性成纤维细胞生长因子 (bFGF)与天然型无机骨 (NNB)复合人工骨修复兔下颌骨缺损的效果 ,以期为临床提供一种较为理想的骨替代材料。方法　在 30只成年新西兰兔双侧下颌骨下缘形成 15mm× 6mm全层骨膜骨质缺损 ,每一缺损作为一个实验单位 ,术后 3周、6周、12周取材行大体标本、X片观察 ,并对HE染色切片行计算机定量分析。结果　复合骨与NNB相比 ,同期成骨量大 ;6周时复合骨成骨面积尚不如自体骨 ,而 12周时已接近自体骨水平 ,空白缺损则始终未能修复。结论　复合骨生物相容性好 ,效果明显优于NNB ,远期与自体骨相似 ,是一种较为理想的骨替代材料。Objective To study the effects of artificial bone composite of basic fibroblast growth factor(bFGF) with natural non organic bone(NNB)in repairing mandibular defects in rabbits,in order to provide an ideal bone replacement material for clinic.Methods 15mm×6mm bilateral mandibular periosteum bone defects were made surgically in 30 New Zealand adult rabbits and each defect as an experimental unit .The defects were randomly repaired with composite bone, NNB, fresh autogenous bone at 3,6,12 weeks after surgery according to the total random design, blank control group was stayed. Specimens obtained were evaluated with general observation, X ray as well as computerized histological microscopic analysis.Results New bone formed and calcified earlier in composite bone and the bone area was greater in the same period than that in NNB. The new bone area in composite bone was still smaller than that of autogenous bone at 6 weeks, while at 12 weeks they had no significant differences. The blank defects were not repaired even in the end.Conclusion Composite bone had sound biocompatibility. It was much better than NNB and can be compared with autogenous bone 12 weeks after operation. So it can be an ideal alternative for bone replacement.

关 键 词：天然型无机骨 碱性成纤细胞生长因子 复合人工骨 颌骨缺损 动物实验 

分 类 号：R782.13[医药卫生—口腔医学]