层粘连蛋白在修复性牙本质形成中的免疫定位  

Immunolocalization of Laminin during Reparative Dentinogenesis

作　　者：陈智[1] 樊明文[1] 张旗[1] 边专[1] 彭彬[1] 

机构地区：[1]武汉大学口腔医学院牙体牙髓科,湖北武汉430079

出　　处：《上海口腔医学》2001年第3期243-245,共3页Shanghai Journal of Stomatology

基　　金：国家自然科学基金 ( 39830 430 ) ;湖北省自然科学基金( 98J0 96 )

摘　　要：目的　研究层粘连蛋白 (laminin ,LN)在牙髓修复中的免疫定位和分布特征。方法　在大鼠第一磨牙制备单面洞 ,分别观察 3d、15d和 30d后修复性牙本质的形成情况。以免疫组化技术检测LN的免疫反应。结果　术后3d ,修复性牙本质尚未形成。牙髓内成牙本质细胞呈阳性染色 ,前期牙本质为弱阳性。术后 15d ,可见修复性牙本质形成和成牙本质细胞样细胞的出现 ,成牙本质细胞样细胞和牙髓细胞免疫染色呈阳性。术后 30d ,已形成的修复性牙本质内LN染色阳性 ,免疫活性特别集中于修复性牙本质与牙髓交界处 ,成牙本质细胞样细胞染色呈强阳性。结论　LN的分布特征提示 ,在修复性牙本质形成过程中 。Objective To investigate the immunolocalization of laminin in the process of dental pulp injury and repair. Methods One cavity was prepared on the mesial surface of the first molars of Wistar rats. The animals were sacrificed in 3, 15, and 30 days of post operation. After histological process, the paraffin sections were reacted with monoclonal antibodies against rat laminin using SABC method.Results Immunolocalization demonstrated positive staining for laminin in the different stages of dental pulp repair. In 3 days group, no reparative dentin were observed. The positive staining was located in odontoblasts and in predentin. After 15 days, reparative dentin and odontoblasts were observed. The odontoblast like cells and dental pulp cells were stained positively. 30 days later, the stronger staining intensity was observed in odontoblast like cells, pulp cells and in reparative dentin. Immunological reaction was particularly located among the interface of reparative dentin and dental pulp.Conclusion The localization feature of laminin suggested that laminin may be a favored factor for the adhesion of dental pulp cell during reparative dentinogenesis.

关 键 词：修复性牙本质 层粘连蛋白 牙髓 免疫定位 

分 类 号：R780.2[医药卫生—口腔医学]