吉兰-巴雷综合征患者人类白细胞抗原A、B的基因分型  

Distribution of HLA-A, B alleles in patients with Guillain-Barré syndrome

作　　者：李春岩[1] 郭力[1] 王维平[1] 刘瑞春[1] 王桂荣[1] 龚燕平[1] 曹宁[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000

出　　处：《中华神经科杂志》2001年第6期336-339,共4页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目 ( 39870 2 6 1) ;河北省自然科学基金资助项目 ( 39935 1)

摘　　要：目的　研究经典型吉兰 巴雷综合征 (GBS)———急性炎性脱髓鞘性多神经根神经病(AIDP)和其亚型———急性运动轴索型神经病 (AMAN)易患性与人类白细胞抗原 (HLA) A、B等位基因分型的关系 ,从免疫遗传学角度探讨自身内在因素在GBS不同亚型发病中的可能作用。方法　以改良快速盐析法自静脉血中抽提基因组DNA ,采用聚合酶链反应 顺序特异性引物法 (PCR SSP) ,结合琼脂糖凝胶电泳对 31例AIDP患者、33例AMAN患者和 132名健康对照进行HLA A、B位点等位基因分型。结果　AIDP组HLA A33频率 (2 2 6 %)较对照组 (4 5 %)升高 ,相对危险度 (RR)为 6 1,校正概率值Pc =0 0 11;AMAN组HLA B35、B15频率 (34 5 %,5 1 7%)较对照组 (6 9%,2 0 8%)升高 ,RR分别为7 1、4 1,Pc分别为 0 0 0 0 8、0 0 15。结论　AIDP和AMAN与健康对照比较 ,有着不同的HLA等位基因分布。HLA A33与AIDP易患性相关联 ,HLA B15、B35与AMAN易患性相关联。推测AIDP和AMAN发病机制、病理改变的差异与在免疫应答、调控等各环节有关键作用的HLA的型别有关。Objective To study the relations between the susceptibility to AIDP and AMAN, two forms of Guillain-Barré syndrome (GBS), and the frequency of HLA-A, B alleles. Methods A case-control research was done in 31 cases of AIDP, 33 cases of AMAN and 132 health individual controls. DNA was extracted from peripheral blood leukocytes by improved fast saltingout. HLA-A, B antigens were typed by DNA-based technology, PCR-sequence specific primers (PCR-SSP) method. In determination of allelic polymorphism by PCR amplification with SSP, oligonucleotide primers are designed to obtain amplification of specific alleles or groups of alleles. Assignment of alleles is based on the presence or absence of PCR amplified product, which may be detected by agarose gel electrophoresis. Results On research of HLA-A, B alleles polymorphism, it showed that HLA-A33 frequency was increased significantly in AIDP patients [22.6% vs 4.5%,corrected probability (Pc)=0.011]; related risk (RR) was 6.1; HLA-B15, B35 frequencies were increased (51.7% vs 20.8%, Pc =0.015; 34.5% vs 6.9%, Pc=0.000 8); RR was 4.1 and 7.1, respectively. Conclusions There are different distribution of HLA-A, B alleles in AIDP and AMAN, two forms of GBS. AIDP susceptibility is associated with HLA-A33, while AMAN is with HLA-B15, B35.

关 键 词：格林-巴利综合征 HLA-A抗原 HLA-B抗原 基因型 

分 类 号：R745.43[医药卫生—神经病学与精神病学]