虎纹蛙病毒主要衣壳蛋白基因的克隆及其序列  

Cloning,sequence analysis of the major capsid protein gene of newly isolated iridovirus from Rana tigrina

作　　者：苗素英[1] 何建国[1] 张利红[1] 曾慷[1] 王晓红[1] 张旻[1] 江静波[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《水产学报》2001年第6期559-563,共5页Journal of Fisheries of China

基　　金：广东省自然科学基金项目资助 (990 2 5 5 )

摘　　要：从新分离感染虎纹蛙的病毒培养细胞中提取病毒DNA作模板 ,用分别对应于蛙病毒 3型 (FV3)主要衣壳蛋白 (MajorCapsidProtein ,MCP)基因读码框两侧的寡核苷酸片段作引物进行PCR扩增 ,得到预期大小基因片段 ,进一步将此基因片段插入到pGEM T载体中 ,进行全长片段的序列测定和分析。结果表明 ,编码虎纹蛙病毒的MCP基因的读码框核苷酸数为 1392bp ,编码 4 6 3个氨基酸 ;基因的核苷酸序列与其他脊椎动物虹彩病毒的MCP基因序列比较结果显示 ,该病毒与蛙病毒属的FV3的同源性 (98% )明显高于囊肿病毒属的FLDV 1(5 2 % ) ,并且与虹彩病毒科其他成员的MCP基因序列均有所不同 。The complete gene of major capsid protein (MCP) of the recently isolated iridovirus from Rana tigrina was amplificated using the primers corresponding to the two sides of the frog virus 3 (FV3) MCP gene and was cloned into the pGEM T easy vector. The MCP gene of the virus was 1 392 bp in size and had much higher identity to FV3 (98%), the type species of the genus Ranavirus, than to lympocystis disease virus (52%), the type species of the genus Lymphocystivirus. Comparison of the MCP sequence here with this of other vertebrate iridoviruses showed that the iridovirus examined here was a new member of the genus Ranavirus.

关 键 词：虎纹蛙 虹彩病毒 衣壳蛋白基因 克隆 病毒分类 

分 类 号：S947.19[农业科学—水产养殖]