以硅胶和Agarose 6B为载体金属螯合亲和层析分离纯化重组人Cu,Zn-SOD  

Purification of rh-Cu,Zn SOD by Metal Chelate Affinity Chromatography Using Silica Gel and Agarose 6B as Carrier

作　　者：黄竹[1] 袁勤生[1] 

机构地区：[1]华东理工大学生物化学研究所,上海200237

出　　处：《药物生物技术》2001年第5期255-259,共5页Pharmaceutical Biotechnology

摘　　要：以层析硅胶Agarose 6B ,制备了Cu2 + 螯合亲和载体 ,利用合成的Cu2 + IDA 硅胶亲和柱和Cu2 + IDA Agarose 6B亲和柱分离纯化了重组人Cu ,Zn SOD ,并比较了这两 2亲和载体的纯化效果和性能。硅胶纯化SOD的比活、纯化倍数和回收率分别为 75 86U/mg·protein、6 .5倍和 86 .7% ;而Agarose 6B纯化SOD的比活、纯化倍数和回收率则分别为 6 2 2 3/mg·protein、5 .8倍和 93.0 %。 2种亲和载体 5次使用后 。Silica gel (for chromatography) and agarose 6B were used as carrier for chromatography, then Cu 2+ was chelated to form metal chelate affinity column. The recombinant human Cu,Zn superoxide dismutase (rh Cu,Zn SOD) was purified in one step metal chelate affinity chromatography by Cu 2+ IDA silica gel column and Cu 2+ IDA Agarose 6B column. Comparison of purification results between silica gel and agarose 6B was discussed. The SOD specific activity, purification fold and yield was 7586U/mg, 6.5 and 86.7% respectively, when using silica gel as carrier, while which were 6223/mg, 5.8 and 93.0% respectively when using agarose 6B as carrier. After using five times by the two carrier, the purification results of SOD did not change.

关 键 词：金属螯合亲和层析 层析硅胶 Agarose6B 重组人Cu Zn-SOD 

分 类 号：R394.8[医药卫生—医学遗传学]