导入AGPase基因的转基因可育水稻及其经济性状的研究  

Studies on Transgenic Rice(Oryza sativa L.)Plants Transformed with AGPase Gene and Its Economic Characters

作　　者：宋敏[1] 李援亚[1] 张云孙[1] 

机构地区：[1]云南大学生物技术系,云南昆明650091

出　　处：《华北农学报》2001年第4期11-14,共4页Acta Agriculturae Boreali-Sinica

基　　金：云南省自然科学基金重点项目 (96C0 0 3Z)

摘　　要：以农杆菌介导法将淀粉合成关键酶AGPase (腺嘌呤二磷酸葡萄糖焦磷酸化酶 )基因导入云南地方优质水稻合系 35 ,38,39,4 1,4 2中 ,获得了大量的可育转基因植株。PCR扩增检测 ,外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明 ,转基因水稻种子的千粒重比对照明显提高 。AGPase gene,the critical starch synthesis enzyme gene,was transformed into high class rice No.35,38,39,41,42 by Agrobacterium mediated transformation and fertile transgenic rice plants were obtained.The results of PCR indicated that the foreign gene had been integrated into the rice genome.The research showed that 1000 grain weight of transgenic plants was obviously improved,but the starch content of the rice was little changed.

关 键 词：AGPase基因 根癌农杆菌 水稻 淀粉含量 千粒重 转基因 品种改良 

分 类 号：S511.035.3[农业科学—作物学]