DNA芯片与应用  

DNA chip technology and applications

作　　者：杨学森[1] 余争平[1] 

机构地区：[1]第三军医大学军事劳动卫生教研室,重庆400038

出　　处：《生物技术通讯》2001年第1期60-62,共3页Letters in Biotechnology

摘　　要：DNA芯片就是利用光导原位化学合成或液相合成自动化点样 ,将数以万计的寡核苷酸固定于固相支持物硅片、尼龙膜上 ,与荧光素或同位素标记的待检样本DNA/cDNA杂交 ,通过对杂交信号分析反映样本中的DNA序列信息。它广泛应用基因表达、DNA测序、基因分型、基因突变与多态性检测和遗传作图等生物医学研究领域。DNA chips are fabricated either by in situ light?directed chemical synthesis or by automatically spotting after conventional liquid synthesis, for binding thousands of oligonucleotide probes with the surface of solid support. Fluorescent or isotope labeled sample DNA/cDNA are hybridized to the chips, and the sequence information is analysised by detecting the hybridization signal. This technology has been successfully applied to monitoring the expression of many thousands of genes, DNA sequencing, gene typing, gene mutation and polymorphism screening, as well as mapping of genome.

关 键 词：DNA芯片 DNA序列信息 应用 

分 类 号：R394.8[医药卫生—医学遗传学] R446[医药卫生—基础医学]