大鼠心脏细胞条件培养基对小鼠ES细胞特性的维持  

The Medium Conditioned Incubation with Rat Heart Cells Maintains the Properties of ES Cells

作　　者：孟国良[1] 滕路[1] 邹冀中[1] 薛友纺[1] 尚克刚[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《Acta Genetica Sinica》2001年第10期911-920,T001-T002,共12页

基　　金：国家 8 63高技术资助项目 ( 8631 0 1 0 78);国家自然科学基金重点项目 (No.39730 2 5 0 )资助~~

摘　　要：以C1 9- 2和MESPU - 1 3为供试细胞 ,用克隆测试、传代培养等方法对 1 7种细胞的条件培养基进行了筛选 ,结果表明 ,大鼠心脏细胞的条件培养基 (RH -CM)具有显著抑制小鼠ES细胞分化、维持其二倍体核型、促进ES细胞贴壁生长的作用。经RH -CM培养 1 0代和 2 0代的小鼠ES细胞在体内外分化能力上仍保留了原ES细胞的多方向分化潜能和特征 ;RH -CM也可作为小鼠ES细胞培养基的添加物 ,用含 70 %RH -CM的ES细胞培养基和小鼠胚胎原代成纤维细胞饲养层 (PMEF)培养ES细胞 ,可长期有效地维持其未分化状态和二倍体核型。RTkinds of conditioned media were selected by plating tests, propagation and other tests with two cell lines, C-19-2 and MESPU-13. The results suggested that the rat heart cells conditioned media (RH-CM) can inhibit the spontaneous differentiation effectively, maintain the normal diploid karyotypes and promote adherence and proliferation of mouse ES cells. The ES cells were propagated in RH-CM to the 20th passage remaining their pluripotent in vivo and in vitro differentiation ability. RH-CM can be used as supplement of ES cell media. ES cells which are cultured in media containing 70% RH-CM and on PMEF feeder can maintain their undifferentiation state and diploid karyotype. RT-PCR detection suggested that there was mLIF expression in rat heart cells.

关 键 词：RH-CM ES细胞系 LIF 嵌合鼠 RT-PCR 培养基添加物 未分化 二倍体核型 

分 类 号：Q953[生物学—动物学]