转SCaM-GFP融合基因烟草中钙调素分泌特性的研究  

Secretion of Calmodulin in Transgenic SCaM-GFP Tobacco

作　　者：周华林[1] 马力耕[1] 刘曼[1] 毛国红[1] 孙大业[1] 

机构地区：[1]河北师范大学分子细胞生物学实验室,石家庄050016

出　　处：《Acta Botanica Sinica》2001年第12期1300-1302,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金重点项目 ( 39730 2 30 );国家重点基础研究规划项目 (G19990 1170 0 );教育部科学技术研究重点项目 ( 0 0 14 0 );国家杰出青年科学基金 ( 30 0 2 5 0 2 4);教育部骨干教师基金~~

摘　　要：The binary vectors which respectively contain chimeric genes (SCaM1-GFP, SCaM4-GFP) were constructed and used to transform tobacco (Nicotiana tabacumL.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.The binary vectors which respectively contain chimeric genes (SCaM1-GFP, SCaM4-GFP) were constructed and used to transform tobacco (Nicotiana tabacum L.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.

关 键 词：大豆钙调素亚型 绿色荧光蛋白 分泌 SCaM-GFP 融合基因 转基因烟草 

分 类 号：Q945[生物学—植物学]