培养条件对嗜水气单胞菌胞外蛋白酶合成与分泌的影响  

Effects of culture conditions on the production of Aeromonas hydrophila extracellular protease

作　　者：储卫华[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095

出　　处：《南京农业大学学报》2001年第3期65-68,共4页Journal of Nanjing Agricultural University

基　　金：国家九五攻关子课题 (96 0 0 5 0 3 0 2 )

摘　　要：对体外培养嗜水气单胞菌的胞外蛋白酶 (ECPase)产生条件进行了优化。结果发现 ,其产量与碳源、氮源、培养温度、培养基初始pH值等有关。蔗糖能促进蛋白酶的产生 ,NH4 + 抑制蛋白酶的产生。其最佳产酶条件为 :pH 7 5的0 0 2mol·L-1KC1,0 0 6mol·L-1K2 HPO4 ,5g·L-1的蔗糖和 5g·L-1的胰蛋白胨水 ,2 8℃ ,15 0r·min-1摇床培养 6 5h。在此条件下酶活性可达 2 0 8U·mL-1。The effects of culture conditions on the production of extracellular proteases(ECPase)by Aeromonas hydrophila(Ah)J?1 strain was conducted. It was found that the production of ECPase was effected by carbon sources,nitrogen sources,temperature,and the initial pH.The production of ECPase was promoted by sucrose and was inhibited by NH 4 +.The optimal medium was as follows:5 g·L -1 sucrose,5 g·L -1 tryptose,0 02 mol·L -1 KCl,0 06 mol·L -1 K 2HPO 4 and pH 7 5.In this case, when Ah J?1 was cultured at 28 ℃ for 65 hours on a shaker with a speed of 150 r·min -1 ,the ECPase production could reach 20 8 U·mL -1 .

关 键 词：嗜水气单胞菌 胞外蛋白酶 培养条件 合成 分泌 病原菌 

分 类 号：S852.6[农业科学—基础兽医学]