抗人脑胶质瘤单克隆抗体H_(12)的荧光标记  

Method of Monoclonal Antibody H_(12) Labelled by Fluorescence

作　　者：庄明华[1] 白晔[2] 高立达[3] 李明远[4] 牟家婉[4] 何俊[5] 李勇[5] 蒋忠华[4] 东云华[4] 

机构地区：[1]汕头大学医学院第二附属医院神经外科,广东汕头515041 [2]汕头大学医学院第二附属医院针灸科,广东汕头515041 [3]华西医科大学第一附属医院神经外科 [4]华西医科大学基础医学院 [5]华西医科大学药学院

出　　处：《汕头大学医学院学报》2001年第4期248-250,共3页Journal of Shantou University Medical College

基　　金：中国博士后科学基金资助项目 (No :5 3 13 )

摘　　要：目的 :探讨H1 2 单克隆抗体的不同浓度对FITC荧光标记结果的影响。方法 :紫外吸收法测定抗体蛋白质浓度 ,透析标记法进行抗体荧光标记 ,并以ELISA法对抗体进行特异性鉴定及效价分析。结果 :标记前后抗体特异性经ELISA鉴定未见明显差异。结论 :①FITC荧光标记前抗体蛋白质浓度应达 (10～ 2 0 )mg mL ,才能得到合适的F P比值。②F P值在 1～ 2之间者较为理想 ,如果过标记的抗体F P >2则可导致非特异性染色的增加 ,增加假阳性率 ;标记不足F P <1则可导致假阴性率增加。③标记后虽可出现特异性下降和蛋白量的丢失 ;但特异性鉴定与标记前未见明显差异。故标记后抗体H1 2Objective:To explore the influence of the concentration of monoclonal antibody H 12 on the result of fluorescence mark.Methods:Dialysis labeled method was used fluorescence(FITC)mark of monoclonal antibody H 12 ,ELISA method was used to identify The specificity and titer of monoclonal antibody H 12 after labeling fluorescence,Ultraviolet absorption method was used to assay the concentration of monoclonal antibody H 12 .Results:Before labeling fluorescence,the concentration of monoclonal antibody H 12 should be 10mg/mL.After labeling fluorescence,the monoclonal antibody H 12 was collected for three parts and F/P ratio was 1 983,1 835 and 1 272,the concentration of monoclonal antibody H 12 was 1 878,1 781 and 1 062mg/mL.The specificity of monoclonal antibody H 12 was not influenced by fluorescence labeled method.Conclusion:The specificity of monoclonal antibody H 12 is not influenced by fluorescence labeled method.Monoclonal antibody H 12 labeled by FITC is practicable to find an effect method to help the surgeon to identify the margin of the malignant glioma during operation

关 键 词：单克隆抗体H12 脑胶质瘤 荧光标记 

分 类 号：R739.41[医药卫生—肿瘤]