n-3脂肪酸、1,6二磷酸果糖和谷氨酰胺改善大鼠移植小肠的吸收功能  

Combined nutritional support with n-3 fatty acids, fructose-1, 6-diphosphate and glutamine enhances absorptive function of post-transplanted sm all bowel in rats

作　　者：伍晓汀[1] 黎介寿[2] 李宁[2] 戴腾昌[3] 

机构地区：[1]华西医科大学附属第一医院普外科,成都610041 [2]南京军区南京总医院解放军普通外科研究所 [3]上海第二医科大学同位素室

出　　处：《中华实验外科杂志》2001年第6期567-568,共2页Chinese Journal of Experimental Surgery

基　　金：教育部回国人员科研启动基金资助项目 (教外司留1 997 436号 )

摘　　要：目的　探讨n 3脂肪酸 (n 3FA)、1,6二磷酸果糖 (FDP)和谷氨酰胺 (GLN)对移植小肠吸收功能的作用。方法　将 196只Wistar大鼠分别作为供、受体行全小肠异位移植 ,手术前后应用n 3脂肪酸和TPN中加入FDP、GLN输注 10d。15N 甘氨酸 ( 15N Gly)灌注移植小肠 ,分析其血浆丰度。结果　15N Gly 1、2和 3h的丰度值均以n 3FA +FDP +GLN组为最高 ,分别是非必需氨基酸 (NEAAs)对照组的 9.0、3.0和 2 .5倍 ,其余各实验组血浆内15N Gly的上升速度及丰度值也明显高于NEAAs对照组 (P <0 .0 5 )。结论　外源性n 3FA、FDP和GLN特殊营养支持可明显改善移植小肠的吸收功能。Objective To investigate the effects and possible me chanism of n-3 fatty acids (n-3FA), fructose-1,6-diphosphate (FDP) and glutamine (GLN) on absorptive function of posttransplanted small bowel in r ats.Methods 196 inbred strain Wistar rats underwent heter otopic small bowel transplantion (SBT) as donors and recipients respectively. n -3FA, FDP and GLN were given via gastrogavage as well as venous infusion with t otal parenteral nutrition (TPN) solution for 10 days before and after surgery. T ransplanted small bowels in living bodys were perfused with^(15)N-glycine ( ^(15)N-Gly). Absorpted ^(15)N-Gly and its plasma abundance were analyz ed.Results n-3FA+FDP+GLN group had the highest abundance volume of 1, 2,3h, 9.0times, which were 3.0times and (2.5)times of nonessential amino acids (NEAAs) respectively in the control group at the sa me time. In other experimental groups, the raising rate and abundance valume of ^(15)N-Gly were much high er than in the NEAAs control group (P<0.05).Conclusion n-3FA, FDP and GLN supplementation for specific nutritional support could sig nificantly enhance glycine absorption and improve the absorptive function of pos ttransplanted small bowel to nutrients.

关 键 词：吸收功能 小肠移植 大鼠 N-3脂肪酸 1 6二磷酸果糖 谷氨酰胺 

分 类 号：R656.7[医药卫生—外科学]