南京市人群NQO1、CYP1A1、mEH基因多态性与肺癌易感性研究  

NQO1, CYP1A1, mEH genotype polymorphisms and susceptibility to lung cancer in Nanjing population

作　　者：尹立红[1] 浦跃朴 林嫔嫔 陈文萍[3] 于力克[3] 

机构地区：[1]南京铁道医学院公共卫生学院,南京210009 [2]台湾省台中医学院毒理研究所 [3]南京市胸科医院

出　　处：《肿瘤》2002年第1期14-16,共3页Tumor

基　　金：铁道部科技基金 (J98Z0 18;J99Z112 );教育部高等学校骨干教师资助计划项目 ( 2 0 0 0 6 5);江苏省社会发展基金资助项目 (BS2 0 0 0 0 34 );国家自然科学基金 ( 30 170 791)

摘　　要：目的　探讨南京市人群中NQO1、CYP1A1、mEH exon3、mEH exon4基因多态性与肺癌易感性的关系。方法　用病例 -对照研究方法 ,收集南京市区原发性肺癌患者 84例 ,其中鳞癌 35例 ,腺癌 49例 ,同时选择对照 84例。采用PCR技术 ,对样本DNA进行NQO1、CYP1A1、mEH exon3、mEH exon4基因型的检测 ,并分析各基因型与肺癌易感性的关系。结果　南京市区人群NQO1、CYP1A1和mEH exon4与肺癌易感性没有明显关系。mEH exon3基因型与肺鳞癌发生有关 ,野生型个体可降低肺鳞癌发病的风险 (OR =0 .32 ,95 %CI:0 .0 0 78～ 0 .63) ,杂合型和突变型个体患肺鳞癌的危险性明显高于野生型个体 (OR=3 .1 ,95 %CI:0 .0 8～ 6 .1 2 ) ;考虑吸烟因素后 ,mEH exon3基因型与吸烟者肺癌发生有关 ,野生型个体可使肺癌发病风险性降低 (OR =0 .1 8,95 %CI:0 .0 6～ 0 .2 9) ,杂合型和突变型个体患肺癌的危险性增高 (OR =5 .66 ,95 %CI:2 .0 1～ 9.30 )。结论　南京市人群中NQO1、CYP1A1、mEH基因的分布情况与国内外的相关报道存在一定差异 ,种族差异、地域不同可能是造成上述基因分布不同的重要原因。南京市人群中mEH exon3基因杂合型和突变型与肺鳞癌发生有关 。Objective Association between NQO1, CYP1A1 and mEH genotype polymorphisms with lung cancer susceptibility in Nanjing population was analysed. Methods Blood samples was collected from 84 primary lung cancer cases and 84 controls in Nanjing. DNA was isolated from whole blood cells with kit. Genotypes were detected with PCR. Results Polymorphisms in mEH Exon3 were associated with lung cancer. Heterozygous and mutant genotypes related positively to the risk of squamous cell carcinoma(OR=3.1, 95% CI:0.08～6.12), wild type genotype showed a low risk effect (OR=0.32, 95% CI:0.0078～0.63). According to the smoking habit, polymorphisms in mEH Exon3 affected the occurrance of lung cancer in smoking individuals. Heterozygous and mutant genotypes related positively to the risk of lung cancer in smoking individuals (OR=5.66, 95% CI: 2.01～9.30); wild type genotype still showed a low risk effect(OR=0.18, 95% CI:0.06～0.29). Conclusion mEH exon3 genotype may be an important genetic factor in causing lung cancer in Nanjing population.

关 键 词：肺肿瘤 肿瘤易感性 NQO1基因 CYP1A1基因 mEH基因 南京 

分 类 号：R734.2[医药卫生—肿瘤]