苯乙酸对胶质瘤C6细胞p16基因的激活作用及意义  

Growth-inhibitory and differentiation-inducing activity of phenylacetate and increased effect of p16 gene on glioma cell C6

作　　者：陈阳[1] 陈宝琴[2] 田宇[3] 

机构地区：[1]天津石化医院外二科,天津300270 [2]吉林省人民医院,吉林长春130021 [3]吉林大学中日联谊医院神经外科,吉林长春130021

出　　处：《中国实验诊断学》2002年第1期16-17,共2页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目 ( 3990 0 15 2 )

摘　　要：目的　观察诱导分化剂苯乙酸对C6胶质瘤细胞增殖分化过程中抑癌基因p16的蛋白水平表达变化的影响。方法　3 H -TdR掺入法测细胞增殖率 ;免疫组化SP法检测p16基因蛋白水平的表达。结果　应用苯乙酸后 ,细胞cpm值降低 ;p16基因胞浆阳性表达显著升高。结论　苯乙酸对胶质瘤细胞存在剂量依赖性抑制 ,增强了抑癌基因p16表达活性 。Abstrait:Objective To determine the growth-inhibitory and differentiation-induing activity of phenylacetate (PA)on glioma cell line C6,to find the change of p16 gene expression.Methods The effect of PA on cell proliferation using 3 H-TdR assay, P16 gene protein expression studied by immunohistochemistry technique.Results At does ranges 0.625?2 5?5 0 nmol/L,PA caused a dose dependent proliferation inhibitory effect. The protein expression of pl6 gene was notably increased by PA.Conclusion PA caused a does-edpendent proliferation inhibitory effect,the expression of p16 gene activity increased by PA.

关 键 词：苯乙酸 胶质瘤 P16基因 细胞增殖 免疫组织化学染色 

分 类 号：R73-36[医药卫生—肿瘤]