组织芯片技术的建立及其在大肠癌的研究  

Tissue microarray technology and its preliminary application in large intestinal Carcinoma

作　　者：张建中[1] 黄英武[1] 刘冬梅[2] 杨利桃[2] 韩瑞刚[1] 王晋芬[2] 岳茂兴[1] 

机构地区：[1]中国人民解放军第306医院,北京市100101 [2]山西省肿瘤医院,山西省太原市030013

出　　处：《世界华人消化杂志》2002年第2期157-160,共4页World Chinese Journal of Digestology

基　　金：全军十五科研基金课题;No.01MA071~~

摘　　要：目的:探讨石蜡包埋组织芯片技术的方法学及其在大肠癌研究中的意义.方法:选取病理科档案材料中44例大肠癌、13例癌旁结肠黏膜和26份大鼠结肠石蜡包埋组织,采用组织芯片仪及手工法制作208点列阵组织芯片,并进行he染色和mib-1抗体免疫组织化学染色.光镜观察并计数mib-1阳性细胞在各组的分布.结果:经染色后组织芯片大部结构完整,每一点阵均有代表性组织形态;mib-1阳性细胞标记指数(li)在中低分化大肠腺癌(50.17%)中明显高于高分化(管状)大肠腺癌(32.38%),而癌旁大肠黏膜li为10.08%.三者之间均具有非常显著性差异(p<0.01).结论:手工法组织芯片制作技术是一种简易可行的方法,在肿瘤病理研究中必将起到重要作用.mib-1阳性细胞标记指数与肿瘤细胞生长活跃程度相关,可作为判断大肠癌生物学行为及其预后的指标之一.AIM:To explore the methodology of tissue microarray (TMA) tec hnology and its application in tumor pathology. METHODS:Beecher tissue arrayer was used to create a 208-dot tis sue microarray, derived from 44 cases of large intestinal adenocarcinoma, 13 par a-cancerous colon tissues and 26 rat intestinal tissues which were formalin-fi xed and paraffin-embedded. Hematoxylin-eosin and immunohistochemical stains we re performed to evaluate the morphology and ki-67 expression in the specimens. The labeling index (LI) was calculated in the different groups of the tissues. RESULTS:Histologic appearance was well preserved in the most dot s of the TMA section. Labeling index of ki-67 in low-differentiated adenocarc inomas (50.17%) was much higher than that in well- differentiated ones (32.38%) and para-cancerous colon mucosa (10.08%). There were significant differences be tween them (P<0.01). CONCLUSION:Manual TMA technique is a simple and easy-doing meth od,which can be widely used in tumor pathology, expression profiling analysis an d genetic investigation of large-scale specimens.Ki-67 labeling index is well correlated with malignant behaviors and can be used to evaluate the patients' p rognosis.

关 键 词：大肠癌 石蜡色埋组织芯片技术 TMA 肿瘤病理学 

分 类 号：R735.34[医药卫生—肿瘤]