重组人白介素-2痘苗病毒对MKN45胃癌细胞感染性及白介素-2蛋白表达  

The infectivity of the recombinant vaccinia virus expressing human interleukin-2 and its effect on MKN45 gastric cancer cells in vitro

作　　者：郜恒骏[1] 朱红音[1] 童菊芳[1] 顾伟齐[1] 任卫平[1] 萧树东[1] 

机构地区：[1]上海第二医科大学仁济医院,上海市消化疾病研究所200001

出　　处：《中华消化杂志》2001年第9期547-549,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目 ( 39770 345 )

摘　　要：目的　体外研究表达人白介素 2 (hIL 2 )的重组痘苗病毒 (vacciniavirus ,VV)对MKN45胃癌细胞的作用。方法　应用分子病毒学同源重组技术构建能表达hIL 2的重组VV(VMJ6 0 1hIL 2 )。用DNA杂交技术鉴定VMJ6 0 1hIL 2 ,并用聚丙烯酰胺凝胶电泳 (SDS PAGE)检测VMJ6 0 1hIL 2基因产物。用VMJ6 0 1hIL 2体外感染MKN45胃癌细胞株 ,探讨其对体外胃癌细胞的作用。结果　VMJ6 0 1hIL 2对体外MKN45胃癌细胞株有较强的感染性 ,VMJ6 0 1hIL 2感染的MKN45胃癌细胞能分泌具有生物活性的hIL 2蛋白。结论　VMJ6 0 1hIL 2的构建与鉴定 ,进一步为胃癌的免疫基因治疗打下了坚实的基础。Objective To study the effect of the recombinant vaccinia virus expressing human interleukin 2 on MKN45 gastric cancer cells in vitro. Methods Recombinant vaccinia virus expressing hIL 2 (VMJ601hIL 2) was constructed by homologous recombination using molecular virology, and VMJ601hIL 2 was detected by DNA hybridization technique and the recombinant gene product was analyzed by SDS PAGE. In addition, MKN45 gastric cancer cells was infected by VMJ601hIL 2 and the effect of VMJ601hIL 2 on the gastric cancer cells was evaluated in vitro. Results hIL 2 protein with biological activity can be secreted by MKN45 gastric cancer cells after heavily infected by VMJ601hIL 2. Conclusions It is one of the crucial steps that VMJ601hIL 2 has been constructed and identified since it forms essential prerequisite for further in vivo gene therapy of gastric cancer.

关 键 词：白介素-2 痘苗病毒 构建 胃癌 蛋白表达 细胞感染性 免疫疗法 

分 类 号：R735.2[医药卫生—肿瘤]