α-干扰素联合重组肿瘤坏死因子体外净化HL-60细胞作用研究  

Experimental study on in vitro purgation of HL-60 cells by interferon combined with recombinant human tumor necrosis factor

作　　者：肖若之[1] 覃雪玲[1] 刘文达[1] 甘丽霞[1] 刘相富[1] 刘桂莲[1] 

机构地区：[1]中山医科大学附属第三医院内科,广东广州510630

出　　处：《中国肿瘤临床与康复》2002年第1期19-21,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　观察α 干扰素 (α IFN)联合肿瘤坏死因子 (TNF)体外净化HL 6 0细胞的效果。方法　不同浓度α IFN及TNF与HL 6 0细胞体外作用 2小时后 ,再做体外集落培养 ,计算清除率。结果　TNF联合α IFN能协同灭活HL 6 0细胞 ,可清除 90 %以上的白血病细胞 ,但对CFU GM的形成却没有协同抑制作用 ,抑制率仅为 35 %左右 ,两者联用的最适浓度是α IFN和TNF均为 10 0U/ml。结论　TNF联合α IFN可用于白血病自身骨髓中残留白血病细胞的净化。Objective To observe the effectiveness of alpha-interferon(α-IFN) combined with recombinant human tumor necrosis factor(rhTNF)on purgation HL-60 cells in vitro.Methods Different concentrations of α-IFN and rhTNF were mixed with HL-60 cells for 2 hours,then cultured it in vitro and calculated clearance rate.Results Combination of α-IFN and rhTNF showed a synergic elimination on HL-60 cells with a celarancer rate of above 90%,but no synergic inhibition on the normal bone marrow CFU-GM9 (inhibitory rate only 35%) was observed.Concentrations of both α-IFN and rhTNF were 100U/ml showed best results. Conclusion α-IFN combined with rhTNF can be used to remove the residual leukemic cells from autologous bone marrow.

关 键 词：干扰素 肿瘤坏死因子 白血病 HL-60细胞 体化净化 

分 类 号：R73-36[医药卫生—肿瘤]