珍香胶囊对人食管癌的抗癌作用及8其机制研究  

ANTITUMOR EFFECT AND MECHANISMS OF ZHENXIANG CAPSULE ON HUMAN ESOPHAGEAL CARCINOMA

作　　者：许锦阶[1] 徐小虎[1] 王德昌[2] 曾宪明[3] 

机构地区：[1]汕头大学医学院病理学和病理生理学系,广东汕头515031 [2]中国医学科学院肿瘤研究所药理室,北京100073 [3]中山医科大学实验动物中心,广东广州510089

出　　处：《癌变．畸变．突变》2001年第3期147-151,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家科技型中小企业技术创新基金资助项目!(99C2 6 114 4 0 0 0 49);广东省中医药管理局科研基金资助项目!(98416 )

摘　　要：目的 :探讨珍香胶囊对人食管癌的抗癌作用及其机制。 方法 :应用光镜、透射电镜和流式细胞仪 ,观察珍香胶囊诱导体外培养的人食管癌细胞株EC10 9细胞凋亡的作用 ;应用人食管癌裸鼠移植瘤模型 ,观察珍香胶囊的抗癌作用。结果 :在体外 ,经珍香胶囊处理的EC10 9细胞出现典型的细胞凋亡特征。在体内 ,珍香胶囊 3个剂量 [4 80 0mg/(kg·bw)、2 40 0mg/(kg·bw)、12 0 0mg/(kg·bw) ]对裸鼠移植的人食管癌的抑瘤率分别为 70 .80 %～ 75 .0 0 % (P <0 .0 1)、5 8.33 %～ 6 4.71% (P <0 .0 1)、37.0 4%～ 45 .10 % (P <0 .0 1) ,阳性对照平阳霉毒 (15mg/(kg·bw) )对裸鼠移植的人食管癌的抑瘤率为 5 1.33 %～ 6 0 .78% (P<0 .0 1)。结论 :珍香胶囊在体外有诱导人食管癌细胞产生凋亡的作用 ,这可能是珍香胶囊抗食管癌作用的重要机制。在裸鼠可耐受的剂量下 ,珍香胶囊对裸鼠移植的人食管癌有明显抗癌作用 ,且随着剂量增加 。Purpose: To investigate the antitumor effect and mechanisms of Zhenxiang capsule(ZXC) on human esophageal carcinoma. Methods: Apoptosis induced by ZXC in human esophageal carcinoma cell line EC109 was investigated by optical microscopy, transmission electron microscopy and flow cytometry. Antitumor effect of ZXC was observed on human esophageal carcinoma xenograft in nude mice. Results: After exposure to ZXC, EC109 cells manifested typical apoptotic features. The tumor inhibiting rates in the groups of ZXC 4 800 mg/(kg·bw), 2 400 mg/(kg·bw) and 1 200 mg/(kg·bw) ranged from 70.80% to 75.00%(P<0.01), 58.33% to 64.71%(P < 0.01)and 37.04% to 45.10%(P<0.01), respectively. The tumor inhibiting rates of the pingyangmycin (15 mg/(kg·bw)) group ranged from 51.33% to 60.78%(P<0.01). Conclusion: ZXC induces apoptosis of esophageal carcinoma cells in vitro, which is possibly the primary mechamism of ZXC inhibition of esophageal carcinoma. ZXC has significant antitumor effect on human esophageal carcinoma transplanted into nude mice at tolerant doses. The antitumor effect increases with dosage.

关 键 词：珍香胶囊 食管癌 实验药理学 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]