紫外线照射后可移植性白血病模型微核研究  

EFFECT OF ULTRAVIOLET IRRADIATION ON THE MICRONUCLEI IN TRANSPLANTABLE LEUKEMIA MODEL

作　　者：万美蓉[1] 陈绍志 

机构地区：[1]徐州医学院附院中心实验室,江苏徐州221002 [2]徐州市儿童医院内科,江苏徐州221005

出　　处：《癌变．畸变．突变》2001年第3期157-160,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：江苏省教委资助课题!(98KJD310 0 0 2 )

摘　　要：目的 :研究可移植性T细胞白血病模型 (L72 12细胞 )在不同剂量紫外线照射后的微核变化。方法 :应用胞质阻断培养法检测L72 12细胞微核。结果 :发现该模型对紫外线敏感 ,当紫外线剂量达 45× 10 -7J/ (s·mm2 )以上时 ,其微核细胞率、微核率均高于对照组 (P <0 .0 5 ) ) ,并且剂量 效应曲线呈Y =a +blgX(P <0 .0 1)。 结论Purpose: To study the micronuclear changes in transplantable T cell leukemia model(L7212 cells) after ultraviolet irradiation. Method: Cytokinesis block(CB) micronucleus method was used. Results: The micronucleated cell and micronucleus frequencies in the CB blocked model(+CB group) were higher than those in the CB control group when the dosage was over 45×10\+\{-7\} J/(s·mm\+2). A dose effect curve showed the relationship of Y=a+blgX(P<0.01). Conclusion: Cytokinesis block micronucleus method is more sensitive than the conventional culture for the measurement of micronuclei induced by ultraviolet irradiation.

关 键 词：白血病 L7212细胞 微核 紫外线照射 

分 类 号：R733.7[医药卫生—肿瘤]