同型半胱氨酸对大鼠器官形成早期卵黄囊内吞功能的影响  

Inhibition on the endocytosis of early visceral yolk sac induced by homocysteine in rats

作　　者：赵如冰[1] 李勇[1] 陈星[1] 赵智榕 

机构地区：[1]北京大学公共卫生学院,北京100083

出　　处：《卫生研究》2002年第1期32-33,共2页Journal of Hygiene Research

基　　金：国家"973"项目 (No .G1 9990 5590 4 );国家自然科学基金资助项目 (No .39870 845)

摘　　要：应用显微解剖和移植术 ,初步建立了 9 5日龄大鼠卵黄囊 (VYS)胎盘体外培养模型 ,并观察了同型半胱氨酸 (HCY)对VYS内吞功能及生长发育的影响。结果显示 :HCY可抑制VYS的内吞功能 ,实验组与对照组相比内吞指数明显下降 (P <0 0 1) ;HCY还可抑制VYS生长及其血管形成 ,实验组的VYS直径明显小于对照组 (P <0 0 1)。结果提示 :VYS对胎盘内吞功能的损伤及对其血管形成分化的抑制作用 ,可能是HCY主要致畸机理之一。The effect of homocysteine (HCY) on the development and endocytosis of rat visceral yolk sac (VYS) was investigated. A preliminary method on early (GD9.5) rat yolk\|sac placenta cultured in vitro was established by techniques using microdissection and transplantation. The results showed that HCY did damage VYS development and blood vessel formation, the damages were characterized by shrunk surface, small or defective blood island and significant reduction of diameter ( P <0.01). HCY also inhibited the process of VYS transport, specifically the endocytosis (as measured by [U- 14 C] sucrose uptake), the rate of endocytosis was significantly decreased when HCY was included in the medium ( P <0.01). These results suggested that the inhibition of sanguimotor development and the reduction of endocytosis induced by HCY may directly correlate with the teratologic and embryopathologic events.

关 键 词：脏层卵黄囊胎盘 同型半胱氨酸 内吞功能 实验研究 

分 类 号：R153.1[医药卫生—营养与食品卫生学] R172[医药卫生—公共卫生与预防医学]