Bt水稻的抗虫性鉴定及转基因的遗传分析  

Identification of Bt Rice for Resistance to Stripe Stem Borer and Genetic Analysis of Transgenes

作　　者：姚方印 朱常香[2] 李广贤 温孚江[2] 

机构地区：[1]山东省水稻研究所,济宁272077 [2]山东农业大学生命科学学院,泰安271018

出　　处：《中国农业科学》2002年第2期142-145,共4页Scientia Agricultura Sinica

基　　金：国家转基因植物研究与产业化开发专项计划课题 (J99 B 0 18);山东省自然科学基金资助课题 (Y98D10 0 5 8)

摘　　要：利用Basta抗性选择技术快速检测Bt转基因水稻及杂交后代群体单株。试验表明 ,阳性单株在室内和田间有良好的抗螟虫性能 ;在杂交后代中抗除草剂bar基因和目的基因cryIA(b)紧密连锁与协同表达 ;外源基因在F2和BC1群体中遵循孟德尔分离规律 ;应用杂交、回交育种方法将抗虫基因转育到推广品种中是可行的。Resistance to Basta has been used to detect Bt transgenic rice and progenies of Bt transgenic rice crossed to rice varieties cultivated in the Huang Huai area. The results indicated that Basta positive plants were highly resistant to stripe stem borer(Chilo suppressalis) in the laboratory and field. cryIA(b)gene inherited and expressed together with the marker gene bar. Mendelian segregation of the marker gene bar was observed in F 2 and BC 1 progenies. This study implicates that it is possible to transfer cryIA(b)gene into other main cultivated varieties through crosses and back crosses.

关 键 词：螟虫 转基因水稻 除草剂 遗传分析 抗性鉴定 BAR基因 BT基因 抗性育种 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]