广东省新城疫病毒地方强毒株的分离鉴定  

The Isolation and Identification of the NDV Virulent Strains of Guangdong

作　　者：秦智锋[1] 贺东生[2] 刘福安[2] 

机构地区：[1]深圳出入境检验检疫局,广州深圳518000 [2]华南农业大学动物医学系农业部养禽与禽病重点开放实验室,广东广州510642

出　　处：《动物医学进展》2002年第1期67-69,共3页Progress In Veterinary Medicine

基　　金：广东省自然科学基金资助(980160)

摘　　要：从广东省各地方分离１０余株地方强毒株，对其中的五株地方强毒株进行了分离鉴定。将各毒株病肝研磨离心后取上清，绒毛尿囊腔接种９～１１日龄的鸡胚。鸡胚接种后３６～４２　ｈ死亡，鸡胚全身出血，含病毒的尿囊液红血球凝集效价（ＨＡ）为１∶２９～１∶２１５，并能被ＮＤ阳性血清特异性抑制，却不能被ＡＩＶ　Ｈ５亚型血清所抑制。用自己设计的特异性检测引物，对五个毒株进行了ＰＣＲ鉴定，可扩增出特异性的目的条带。再结合临床症状和剖检病变，可初步判定为新城疫病毒。为以后进行广东省新城疫病毒分子流行病学研究打下了基础。Ten strains of virulent Newcastle Disease Virus (NDV) f rom Guangdong were isolated though chicken embryo inoculating and the harvested infectious allantoic fluid was tested by HA assay. The titers attained 1:29-1: 215 and the HA activity could be inhibited with antiserum against NDV.One step R T-PCR was employed to amplify a cDNA fraction (including for four of the five h emagglutinin determinants) of HN gene of five strains using a pair of specific p rimers,which indicated the isolations were NDV strains.

关 键 词：新城疫病毒 PCR 分离 鉴定 广东地方株 毒强株 

分 类 号：S852.659.5[农业科学—基础兽医学]