抗人p185单克隆抗体的体外直接抗癌效应  

Investigation on the anti-cancer activities of anti-p185 monoclonal antibodies in vitro

作　　者：李平[1] 李昀[1] 王琛[1] 刘兢[1] 

机构地区：[1]中国科学技术大学生命科学学院细胞免疫实验室,合肥230027

出　　处：《中国免疫学杂志》2002年第1期33-35,共3页Chinese Journal of Immunology

基　　金：安徽省科委基金资助项目(No.99-D050)

摘　　要：目的:对使用“表面表位包埋法”构建成功的抗人肿瘤抗原p185的单克隆抗体(mAb)的抑癌活性进行研究。方法:采用免疫荧光染色法、MTT法、软琼脂培养法及细胞长期增殖法等,测定3株mAb的对高表达p185的乳腺癌细胞及转染p185基因的成纤维细胞的抑制活性,以及mAb对提高癌细胞的化疗药物敏感性的效应。结果:这3株mAb均可特异性地明显抑制癌变细胞的生长,并不同程度上提高癌细胞对化疗药物的敏感性。结论:这几株mAb具有治疗p185高表达肿瘤的应用前景。To study the anti-cancer activity of the three anti-oncoprotein p185 mAbs established by surface epitope masking method so as to provide some basic information for clinical tumor targeting therapy.Methods: Immnune flurorence staining, MTT assay, soft-ar-gar culture and long-term cell proliferation assay detected the suppressive activities of the mAbs against the breast cancer cells overexpressing p185 and p185-transfected fibroblast cells, and the inducing effects of the mAbs on tumors sensitivity to chemotherapy.Results:The 3 mAbs specifically suppressed tumor cell proliferation and enhanced the sensitivity of tumors to chemotherapeutics. Conclusion:These mAbs have the potentials to develop into the clinical tumor targeting therapeutic reagents of the tumors overexpressing p185.

关 键 词：P185 单克隆抗体 乳腺癌 抗癌效应 

分 类 号：R737.9[医药卫生—肿瘤] R730.5[医药卫生—临床医学]