骨髓基质成骨细胞-松质骨基质复合人工骨皮下移植成骨作用的实验研究  

Experimental study on subcutaneous bone formation by marrow stromal osteoblast-cancellous bone matrix compound artificial bone

作　　者：陶凯[1] 毛天球[1] 陈富林[1] 杨维东[1] 顾晓明[1] 陈书军[1] 

机构地区：[1]第四军医大学口腔医学院组织工程实验室,西安710032

出　　处：《中华口腔医学杂志》2002年第1期18-24,T004,共8页Chinese Journal of Stomatology

基　　金：国家"973计划"基金资助项目 ( 19990 5 430 8)

摘　　要：目的　观察骨髓基质成骨细胞 松质骨基质复合人工骨皮下移植的成骨作用 ,探索骨髓基质成骨细胞和松质骨基质分别作为骨组织工程种子细胞和支架材料的可行性。方法　成年新西兰兔骨髓细胞体外培养、诱导后 ,经混匀、接种、固化 ,形成骨髓基质成骨细胞 藻酸盐 松质骨基质复合人工骨 ,并植回取材兔背部皮下 ,对照组分别植入藻酸盐 松质骨基质复合物和松质骨基质。植入 4、8周取材 ,行X线摄片、组织学检查和组织学定量分析 ,观察骨形成情况。结果　骨髓基质成骨细胞 藻酸盐 松质骨基质复合人工骨皮下成骨效果明显优于藻酸盐 松质骨基质复合物组和松质骨基质组。复合人工骨标本兼有膜内成骨和软骨成骨 ,以膜内成骨为主 ;对照组仅见少量软骨成骨。结论　采用组织工程方法形成的复合人工骨皮下成骨作用明显 ,骨髓基质成骨细胞和松质骨基质可以分别作为种子细胞和支架材料而用于组织工程骨的构建。Objective To investigate the feasibility of using marrow stromal osteoblast(MSO) as bone derived cell and using cancellous bone matrix(CBM) as scaffold for bone tissue engineering, the subcutaneous osteogenesis of MSO CBM compound artificial bone(MCCAB) was observed in the experiment Methods The marrow stromal cells of adult New Zealand rabbits cultivated and induced in vitro were used to form MCCAB by mixing,seeding and solidifying methods assisted by alginate The MCCABs were auto transplanted subcutaneously into the rabbits for 4 to 8 weeks The alginate cancellous bone matrix composites or the cancellous bone matrix alone were implanted as control The effectiveness of bone formation was assessed by means of roentgenography, histology and computerized histomorphometry Results The osteogenesis of MCCABs was better than that of the alginate cancellous bone matrix composites and of the cancellous bone matrixes In the MCCABs, both intramembranous and cartilaginous osteogeneses were seen but the former was obvious In the control, only slight cartilaginous osteogeneses were seen Conclusions The osteogeneses of the MCCABs constructed by using tissue engineering method were obvious when transplanted subcutaneously The MSO and CBM can be used as good bone derived cell and scaffold material respectively for tissue engineered bone construction

关 键 词：骨髓细胞 骨移植 组织工程 人工骨 动物实验 MSO CBM MCCAB 

分 类 号：R782.2[医药卫生—口腔医学]