IL-6表达蛋白与mRNA转录在咬合力影响大鼠牙周组织改建中的变化及意义  

Expression of IL-6 and it′s mRNA transcription in the process of rat periodontium remodeling influenced by various bite forces

作　　者：袁林[1] 赵云凤[1] 周伟东[2] 

机构地区：[1]四川大学华西口腔医学院修复科 [2]第一军医大学珠江医院

出　　处：《中华口腔医学杂志》2002年第1期54-57,T008,共5页Chinese Journal of Stomatology

摘　　要：目的　探讨白细胞介素 6 (interleukin 6 ,IL 6 )在不同咬合力状态下对牙周组织改建影响的分子机理。方法　选用Wistar大鼠建立正常咬合力、咬合力废用及代偿的动物模型 ,采用免疫组化和原位杂交的方法 ,观察不同咬合力状态下IL 6蛋白与mRNA在牙周组织细胞中表达的动态变化。结果　咬合力废用组 ,观察到IL 6蛋白与mRNA在牙周组织细胞中的表达较正常咬合力组明显增强 ,代表其染色强度的灰度值分别从 6 5 886± 1 6与 11 8337± 2 8增加到 38 8381± 9 5和 5 7 2 6 2 3±13 6。其形态学显示牙周膜结构稀疏 ,牙槽骨吸收。咬合力代偿组 ,观察到IL 6蛋白与mRNA在牙周组织细胞中的表达也较正常咬合力组明显增强 ,代表其染色强度的灰度值分别从 6 3119± 1 5与11 7714± 2 8增加到 45 5 45 6± 11 1和 81 9391± 19 5。其形态学显示牙周膜结构致密 ,牙槽骨除有吸收外 ,还有新骨形成。结论　IL 6蛋白与mRNA在牙周组织细胞中的表达随着咬合力的改变出现一定的规律性变化 ,提示IL 6在咬合力影响牙周组织改建的过程中起着重要的调节作用。Objective To study the expression of IL 6 in periodontal ligament fibroblasts (PLF)and osteoblasts in order to explain the molecular mechanism of the bite forte on periodontium remodeling.Methods A rat model of different bite forces was established to study the expression of IL 6 and IL 6 mRNA in the PLF and osteoblasts with immunohistochemical (IHC)and in situ hybridization (ISH)techniques Results In the group of disuse bite force, the expression of IL 6 and IL 6 mRNA in the PLF and osteoblasts enhanced apparently compared with that in the group of normal bite force. The gray level values enhanced respectively from 6 5886±1 6 to 38 8381±9 5 and from 11 8337±2 8 to 57 2623±13 6 The loosely arrangement of periodontal fibers and the resorption of alveolar bone were observed in histological study In the group of compensation bite force,the same result was observed with IHC and ISH, the gray level values enhanced respectively from 6 3119±1 5 to 45 5456±11 1 and from 11 7714±2 8 to 81 9391±19 5, but the increase of periodontal fibers,the resorption and formation of alveolar bone were observed simultaneity in histological study Conclusions With the change of bite force,the expression of IL 6 and IL 6 mRNA in PLF and osteoblasts presents a certain regulation.IL 6 may play important roles in the effect of bite force on periodontium remodeling.

关 键 词：白细胞介素-6 牙周膜 咬合力 mRNA 大鼠 口腔正畸 义齿 

分 类 号：R783.6[医药卫生—口腔医学]