pIRVP3/pIRHNVP3核酸表达质粒的构建及对肿瘤细胞的影响  

Construction of nucleic acid expressing vaccines of pIRVP3/pIRHNVP3 and its effects on the tumor cells

作　　者：龚伟[1] 薛立娟[1] 孙大辉[1] 王宏伟[1] 葛涛[1] 罗琴芳[1] 李萍[1] 金宁一[1] 

机构地区：[1]解放军军需大学军事兽医研究所病毒室,吉林长春130062

出　　处：《免疫学杂志》2002年第1期30-32,共3页Immunological Journal

基　　金：国家"973"资助项目 (G1990 1190 2 )

摘　　要：目的　研究应用凋亡素基因 (VP3)和NDVHN基因联合抗肿瘤的可能性。方法　以pIRES1neo为表达载体构建了含鸡贫血病毒VP3基因和长春株HN基因的pIRVP3/pIRHNVP32个核酸重组质粒。将 2个重组质粒在体外转染HeLa和OS 732细胞 ,用荧光显微镜、DNA琼脂糖电泳及TUNEL染色等方法 ,检测凋亡素基因和HN基因导致细胞死亡的类型。结果pIRVP3和pIRHNVP3转染HeLa和OS 732细胞 4 8h后 ,pIRVP3和pIRHNVP3均具有很强的诱导肿瘤细胞凋亡的能力 ,且pIRVP3的抗肿瘤作用强于pIRHNVP3。结论　在体外 。Objective To explore anti tumor effect of apoptin (VP3) and NDV HN. Methods Two nucleic acid recombination vaccines, pIRVP3/pIRHNVP3 were constructed and were transfected to HeLa and OS 732 cells. The mode of cell death was detected by fluorescence microscope, gel electrophoresis and TUNEL assay. Results Both pIRVP3 and pIRHNVP3 could induce apoptosis after HeLa and OS 732 cells were transfected. The effect of anti tumor responses of pIRVP3 was stronger than that of pIRHNVP3. Conclusion pIRVP3/pIRHNVP3 may become a new anti tumor biological product. [

关 键 词：凋亡素 NDV HN 抗肿瘤作用 pIRVP3/pIRHNVP3 免疫治疗 核酸 生物制剂 

分 类 号：R73-36[医药卫生—肿瘤]