新鲜羊膜移植术对角膜新生血管抑制作用的实验研究  

Effects of fresh amniotic membrane transplantation on restraining corneal neovascularization

作　　者：李线 丁琦[2] 陈剑[2] 黄菊天 徐锦堂[2] 

机构地区：[1]深圳市人民医院眼科,广东省518020 [2]暨南大学医学院第一附属医院眼科,510632

出　　处：《眼科新进展》2001年第6期407-409,共3页Recent Advances in Ophthalmology

基　　金：广东省医学科技研究基金资助 (编号:19992 98)~~

摘　　要：目的　探讨新鲜羊膜移植术对角膜碱烧伤后角膜新生血管和上皮愈合作用的影响 ,并与保存羊膜作比较。方法　用 48只新西兰大白兔制作角膜碱烧伤模型 ,烧伤后 48h设立新鲜羊膜移植组 (f AMT)、保存羊膜移植组 (p AMT)和对照组 (C)。术后每日观察角膜新生血管和上皮愈合情况。并于术后 14、30 d以计算机图像分析系统定量测定新生血管和上皮愈合面积。结果　术后 30 d f AMT组、p AMT组、C组角膜新生血管面积分别为87.87m m2 ± 3.5 7mm2 、96 .6 6 mm2 ± 12 .0 0 mm2 、10 5 .13m m2 ± 6 .70 m m2 。统计分析显示 ,f AMT组角膜新生血管面积小于 p AMT组 (P <0 .0 5 ) ,上述 2组均小于 C组 (P <0 .0 5 )。结论　新鲜羊膜移植术可抑制碱烧伤后角膜新生血管生长 ,其作用优于保存羊膜移植术。Objective To probe into the effects of fresh amniotic membrane transplantation on restraining neovascularization and promoting epithelial healings in corneal alkali burns.Methods Corneal alkali-burn models of 48 New Zealand white rabbits were made. The rabbits were divided into fresh amniotic membrane transplantation group (fAMT), preserved amniotic membrane transplantation group (pAMT) and a control group (C). Fourteen and 30 days after operation, the neovascularization and epithelial healings were measured by using a computer image analysis system. Results Thirty days after operation, the areas of corneal neovascularization in fAMT , pAMT and C were respectively 87.87mm 2±3.57mm 2,96.66mm 2±12.00mm 2 and 105.13mm 2±6.70mm 2.Less neovascularization was found in fAMT than that in pAMT,and both of them were less than that in C group(P<0.05). Conclusion Fresh amniotic membrane transplantation has stronger effect on restraining corneal neovascularization than that of preserved amniotic membrane transplantation in corneal alkali burns.

关 键 词：羊膜移植 碱烧伤 角膜上皮缺损 新生血管 

分 类 号：R779.65[医药卫生—眼科]