Argon-Plasma对人工角膜支架材料组织相容性的影响  

Response of cornea to Argon-Plasma in three different materials

作　　者：吴欣怡[1] 盛新华[1] 陈璇[1] 李淑卿[1] 蔡可丽[1] 

机构地区：[1]山东大学齐鲁医院眼科,山东省济南市250012

出　　处：《眼科新进展》2001年第5期313-316,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号 :39970 779);卫生部科研基金立项课题( 98-2 -15 8);山东省科委资助课题( 1999BBCJA3)~~

摘　　要：目的　探讨 3种人工角膜支架材料经 Argon- Plasm a处理后植入兔角膜对兔角膜组织的影响。方法　选用 3种支架材料 :材料 1:聚丙烯和聚丁烯 (80∶ 2 0 )化合而成的多孔材料 ;材料 2∶ Polyester多孔材料 ;材料 3:Expended polytetrafluoroethglene。上述 3种材料切成直径 6 mm的圆盘状 ,每种材料分为处理组和非处理组。处理组材料经 Argon-Plasma处理。选用 6 3只新西兰大白兔 ,共分为 7组 ,每组 9只 ,6组为实验组及对照组 (3组为处理组 ,3组为非处理组 ) ,1组为手术对照组。以显微手术的方法 ,将盘状支架材料植入囊袋内 ,手术对照组仅制备囊袋不植入材料。术后定期观察角膜水肿及新生血管的生长情况。术后 2 8、42、84d取角膜进行角膜组织学及免疫组织化学分析。结果　当材料经处理后角膜水肿较未处理明显减轻 ,8周后 3种材料中角膜水肿均消失。材料 1、2植入时新生血管出现延迟 ,范围也较小 ,同时炎细胞浸润明显减少。术后 6周纤维细胞迁徙入材料内 ,术后 12周空腔填充大量纤维细胞。材料处理与否不影响胶原蛋白的沉积。结论　 Argon-Plasma处理可明显减轻兔角膜炎症反应 。Objective To explore the biocompatibility of three porous materials treated with Argon Plasma which were implanted into the rabbits cornea. Methods Three porous materials were chosen: a melt blown microfiber web of polybutylene: polyproplene (80∶20);Polyester spun laced fabric, and an expended polytetrafluoroethylene. Fifty percent of each material was modified using argon plasma treatment. Discs of 6mm diameter were inserted into interlamellar stromal pockets and followed up for a period of 12 weeks. Clinical examinations were performed weekly.Results Pretrement of the discs reduced corneal edema and neovascularization. The presence of the collagen in the disc indicated that cells had migrated into the disc and deposited a cellular matrix in all these materials.Conclusion Argon Plasma treatment increase the biocompatibility of all materials. Fibroplasia occurred most rapidly into the polyester discs but was accompanied by a large number of inflammatory cells. The material did not alter the distribution of collagen.

关 键 词：人工角膜 组织相容性 Argon-Plasma 动物实验 

分 类 号：R772[医药卫生—眼科]