可诱导结瘤基因nodA的体外转录  

In vitro Transcription of Inducible nod Gene nodA

作　　者：张磊[1] 洪国藩[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《生物化学与生物物理学报》2002年第1期73-76,共4页

基　　金：国家攀登计划资助项目 (No .910 2 310 0 2 )~~

摘　　要：报道结瘤基因nodA的体外诱导转录 :当转录体系中有一定量的调控蛋白NodD和植物诱导分子柚皮素 (naringenin )存在时 ,在体外转录的产物中始终存在着与体内转录相一致的特异产物。体外诱导转录表明 ,可诱导结瘤基因nodA在体内转录时 ,可能同样存在NodD蛋白。We observed in vitro transcription of nodA, an inducible nod gene. Primer extension analysis of the transcripts showed there always existed a transcription product identical to that of its in vivo transcription, when 10 μmol/L naringening and NodD protein was added in the in vitro transcription system. Induced in vitro transcription of nodA gene suggests that in its in vivo transcription there may also exist a kind of physical interaction among NodD protein, inducer naringening and promoter of nodA gene.

关 键 词：可诱导结瘤基因 体外转录 诱导 nodA 根瘤菌 

分 类 号：Q785[生物学—分子生物学]