磷脂酶C抑制剂对脑缺血大鼠脑细胞膜游离脂肪酸释放的影响  

Effect of Phospholipase C Inhibitor on the Free Fatty Acids Liberation in the Focal Ischemic Brains in the Rats Models

作　　者：鲁佑瑜[1] 秦震[2] 莫谷强 

机构地区：[1]上海市第一人民医院神经内科,上海200080 [2]复旦大学附属华山医院神经病学研究所,上海200040 [3]复旦大学药学院色谱中心,上海200032

出　　处：《复旦学报（医学版）》2002年第1期42-44,共3页Fudan University Journal of Medical Sciences

摘　　要：目的　观察磷脂酶C抑制剂苯甲基磺酰氟 ( phenylmethylsulfonylfluoride ,PMSF)对脑缺血时脑细胞膜游离脂肪酸 (freefattyacid ,FFA)释放的治疗及保护作用。方法　选用雄性SD大鼠局灶性脑缺血模型 ,气 液色谱分析技术观察了脑缺血前后应用PMSF对不同缺血时间点脑细胞膜FFA含量的变化。结果　PMSF主要抑制缺血早期磷脂酰肌醇和缺血后期磷脂酰乙醇胺、磷脂酰胆碱的FFA释放。结论　多不饱和脂肪酸 (C2 0∶4、C2 2∶6 )积聚是反映缺血性脑损伤程度的重要指标 。Purpose: To examine the effect of a phospholipase C inhibitor(phenylmethylsulfonyl fluoride, PMSF) on the neuronal membrane free fatty acid(FFA) during cerebral ischemia in the models of rats. Methods: Before and after operation treated with PMSF, using the model of focal cerebral ischemia in SD rats, the FFA contents in the neuronal membranes were measured in the sham-operated group at 1,5,15,60 and 360 min, examing the content of free fatty acid(FFA) with gas liquid chromatography(GLC) technique. Results: PMSF inhibited the release of FFA primarily from phosphatidylinositol at the earlier stage of cerebral ischemia and from phosphatidylcholine and phosphatidylethanolamine at the latter stage of ischemia. Conclusions: The polyunsaturated fatty acids(C20:4, C22:6) during ischemia is a hallmark of the evolution of ischemic brain damage. The results of the present study supported the existence of certain protective and theraprutic effect of phosphlipase C inhibitor against cerebral ischemia.

关 键 词：脑缺血 磷脂酶C抑制剂 脂肪酸类 大鼠 PMSF FFA 

分 类 号：R743.31[医药卫生—神经病学与精神病学]