青光眼术后滤过区生长因子的表达及干扰素拮抗生长因子作用的初步研究  

The Expression of Growth Factors in Filtering Area Following Sclerectony and the Interaction between Growth Factors and Interferon

作　　者：张秀兰[1] 梁凌毅[1] 彭大伟[1] 杨培增[1] 郑湖玲[1] 周红颜[1] 

机构地区：[1]中山医科大学中山眼科中心,广州510060

出　　处：《眼科学报》2001年第2期106-110,共5页Eye Science

基　　金：广东省自然科学基金(940201);广州市重点科技攻关项目基金(1999-z-101-04)资助

摘　　要：目的:探讨青光眼术后滤过区局部生长因子的表达及干扰素α-2b（interferon alpha-2b,IFNα-2b）在体内环境下对b成纤维生长因子（beta-Fibroblastic Growth Factor,bFGF）、转化生长因子β1（Transforming　Growth　Factor　betal,TGFβ1）的作用。方法:采用冰冻切片的免疫组化染色技术。标本取自兔眼行巩膜咬切术后的滤过区组织,实验分为正常对照组和用药组两组,各4只兔8只眼。全部兔眼局麻下行标准统一的巩膜咬切手术。用药组于术后当时、术后隔天接受滤过区旁结膜下注射干扰素α-2b 5×105IU0.2ml各1次,正常对照组不用药。分别检测术后第3、5、7、14d滤过区标本内bFGF、TGFβ1抗原的表达。结果:正常对照组标本中,术后3d的滤过区细胞间质已能检测出bFGF或TGFβ1阳性表达产物及阳性细胞;第5至7d达到高峰;第14d的明显减少。用药组的标本中,表达两种生长因子的物质和细胞显著减少。结论:抗青光眼术后手术区局部“沐浴”着生长因子,术后伤口愈合的发生有bFGF、TGFβ1生长因子的参与。而INFα-2b体内环境下具有拮抗bFGF、TGFβ1的作用。眼科学报2001;17:106～110。Objective: To study the expression of beta-Fibroblastic Growth Factor (bFGF) andTransforming Growth Factor betal(TGFβ1) in filtering area following sclerectomy and the interaction between growth factors and interferon alpha-2b(IFNα-2b).Methods: Immunohistochemical technique were applied to test the expression bFGF and TGFβ1 in frozen sections of filtration area following sclerectomy on postoperative daythree, five, seven and 14 in white rabbits, respectively. 16 eyes of eight rabbits in allwere randomly and equally divided into two groups. The exprimental eyes hadsubconjunctivally been administered IFNα-2b 5×105 IU 0. 2ml each time at filteringbleb following operation whereas the control eyes hadn' t taken.Results: In control group, positive cell and substance expressing bFGF and TGFβ1 onpostoperative day three were revealed in the filtration area, on postoperative day five andseven, they were gradually increased. On the postoperative day 14, all significant cellsand substance markly decreased. In experimental group only small amount of positivecell and bFGF or TGFβ1-expressing substance were sparely distributed in the filtration area.Conclusion: The filtration area after glaucoma filtering surgery was bathed in growthfactors such as bFGF and TGFβ1 which well-known play an important role in stimulating wound healing response, IFNα-2b had an inhibitory effect on bFGF or TGFβ1 expression in wound environment. Eye Science 2001; 17: 106- 110.

关 键 词：青光眼 滤过性手术 生长因子 干扰素 

分 类 号：R775[医药卫生—眼科]