实验性自身免疫性脑脊髓炎大鼠模型的建立  

The Generation of Experimental Autoimmune Encephalomyelitis Model in Wistar Rats

作　　者：周璟[1] 张家胜[2] 马宝骊[1] 王利[1] 张继英[1] 柏峻[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025 [2]复旦大学医学院手外科研究所

出　　处：《上海第二医科大学学报》2002年第1期16-18,25,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的为深入研究人类多发性硬化症建立EAE大鼠模型。　方法采用双后腿足垫皮内注射豚鼠全脊髓匀浆 (GPSPH) -福氏完全佐剂 (CFA)混合乳剂 ,辅以注射百日咳疫苗 ,诱导Wistar大鼠EAE模型。取对照及发病高峰期大鼠脑、脊髓组织 ,常规病理切片 ,HE染色。ELISA法检测血清中抗MBP抗体。　结果一次性双后腿足垫皮内注射GPSPH -CFA混合乳剂可诱导Wistar大鼠EAE模型 ,临床症状约在造病后 10～ 15d出现。同时注射百日咳疫苗能提高发病率和发病程度。EAE大鼠脑、脊髓组织HE染色见大量淋巴细胞浸润 ,在血管周围形成淋巴鞘 ,但未见脱髓鞘改变。与对照组相比 ,EAE大鼠血清中抗MBP抗体明显增高。　结论非敏感品系Wistar大鼠可用于制备EAE动物模型。发病大鼠除有EAE临床症状外 。Objective In order to study multiple sclerosis, we developed experimental autoimmune encephalomyelitis (EAE) model in Wistar rats. Methods Wistar rats developed EAE when immunized with both guinea pig spinal cord homogenate(GPSCH) in CFA and pertussis vaccine(PV). The brainstem and spinal cords were removed 10d postimmunization , fixed, embedded , sectioned and stained with hematoxylin and eosin. Antibodies to MBP were detected by ELISA. Results The acute stage of EAE in the Wistar rats occurred 10 ～ 15d postimmunization by a single intradermal injection of GPSCH in CFA. The administration of PV could increase the incidence and severity. Histopathologic results of CNS from EAE rats showed the brainstem and spinal cords had multiple perivascular mononuclear cell infiltrates, without demyelination. The level of antibodies to MBP in the sera obtained from EAE rats was higher compared with control rats. Conclusion EAE could be induced in less susceptible strains such as Wistar rats. The susceptive rats had clinical maufestations such as tail and hindlimp paralysis, inflammatory alterations in CNS and high level antibodies to MBP in sera.

关 键 词：实验性自身免疫性脑脊髓炎 WISTAR大鼠 多发性硬化症 动物模型 

分 类 号：R744.5[医药卫生—神经病学与精神病学]