槲皮素对白血病细胞中PML基因及蛋白表达的影响和定位的研究  

Study of the effects of quercetin on PML gene and protein expression and localization in leukemia cells

作　　者：钟璐[1] 陈芳源[1] 韩洁英[1] 邵念贤[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院血液科,上海血液学研究所白血病研究室,200001

出　　处：《中华血液学杂志》2002年第2期87-90,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 ( 36 970 32 8)

摘　　要：目的　探讨PML基因及蛋白在白血病细胞中的表达和细胞内分布定位。方法　经全反式维甲酸 (ATRA)、槲皮素处理后的NB4、HL 6 0、K5 6 2细胞为研究对象 ,细胞形态学观察采用Wright染色法及荧光染色法 ;PML蛋白细胞内分布定位采用免疫荧光技术 ;PMLmRNA表达的检测采用RT PCR法。结果　①ATRA处理后 ,NB4和HL 6 0细胞形态学上出现分化表现 ,K5 6 2细胞无变化 ;经槲皮素处理后 ,各组细胞形态学上均出现凋亡特征性改变。②ATRA处理后 ,NB4细胞内融合蛋白降解 ,PML蛋白恢复定位 ,HL 6 0、K5 6 2细胞内PML蛋白定位分布无变化 ;槲皮素处理后NB4细胞内融合蛋白降解 ,PML蛋白聚积于POD结构 ,随后降解 ,在HL 6 0及K5 6 2细胞 ,PML蛋白发生相似改变。③ATRA、槲皮素对各组细胞PMLmRNA表达均无显著影响。结论　在不同诱导剂刺激下 ,PML基因在蛋白水平参与细胞终末分化及诱导白血病细胞凋亡机制 ;PML蛋白在POD中发挥诱导凋亡。Objective To investigate PML gene and protein expression and localization in leukemia cell lines. Methods Cell morphology was assayed by Wright and fluorescence stain, PML mRNA expression by RT-PCR, and PML protein localization by immunofluorescence. Results ①Differentiation was observed by morphology in NB4 and HL-60 cells after treatment with all-trans retinoic acid (ATRA) while K562 cells did not show. Apoptosis was found in each cell line after treatment with quercetin. ②After treatment with ATRA, the fusion protein disappeared and PML protein resumed in NB4 cells, while in HL-60 and K562 cells there was no difference from control cells. After treatment with quercetin, the fusion protein disappeared in NB4 cells, then degraded, and so did in HL-60 cells and K562 cells. ③The expression of PML mRNA had no change in all the three cell lines after treatment with ATRA or quercetin. Conclusion PML plays a role of differentiation and apoptosis induction in leukemia cells at the translational level. PML in POD plays a role of apoptosis induction and growth control of leukemia cells.

关 键 词：PML基因 全反式维甲酸 槲波素 细胞株 NB4 HL-60 白血病 

分 类 号：R733.7[医药卫生—肿瘤]