基因重组碱性成纤维细胞生长因子对大鼠视网膜神经节细胞的影响  

Effects of recombinant basic fibroblast growth factor on retinal ganglion cells of rats

作　　者：李校堃[1] 胡爱莲[2] 郑远远[2] 许华[1] 姚成灿[1] 

机构地区：[1]暨南大学医药生物技术研究开发中心,广东广州510632 [2]北京市眼科研究所,北京100730

出　　处：《中国病理生理杂志》2002年第2期147-149,共3页Chinese Journal of Pathophysiology

基　　金：国家"九五"科技攻关项目 ( 96-C0 2 - 0 1- 0 3 )

摘　　要：目的 :研究基因重组碱性成纤维细胞生长因子 (rbFGF)对大鼠视网膜神经节细胞 (RGCs)的影响。方法 :大鼠在视神经部分损伤后 ,球后注射生理盐水、维生素B12 、rbFGF ,伤后 4周进行轴突定量、视网膜神经节细胞定量以及RGCs凋亡的检测 ,观察视神经损伤修复情况。结果 :伤后 4周时 ,生理盐水和维生素B12 对RGCs无挽救作用 ,80 0U、160 0U和 2 4 0 0U的rbFGF对RGCs挽救率分别为 2 4 5 %、2 7 3 %、2 8 5 % ,80 0UrbFGF组、160 0UrbFGF组和2 4 0 0UrbFGF组未发生溃变的轴突数分别是损伤未治疗组的 2 0 3、2 4 3、2 3 1倍。流式细胞仪检测结果显示 :rbFGF治疗 7d后 ,RGCs凋亡率显著减少。结论 :rbFGF可提高视网膜神经节细胞的存活率 ,减少轴突溃变 ,有抗凋亡作用 。AIM: To investigate the pharmacological effects of recombined basic fibroblast growth factor (rbFGF) on retinal ganglion cells (RGCs) of rats. METHODS: Using calibrated cross-action forceps a moderate crush injury was inflicted on the nerve. After crush injury, rbFGF, saline and VB 12 were administered by retrobublar injection. Four weeks after injury , the apoptosis of RGCs was measured with flow cytometer. RESULTS: Four weeks after operation, it was shown that the rbFGF, but not saline or VB 12 injection could significantly improve the maintainance of RGCs of rats. After 800 U, 1600 U and 2400 U rbFGF injection, the injured RGCs were rescued by 24.5%, 27.3% and 28.5% respectively. Furthermore, it was also found that rbFGF injection could effectively prevent the axons from injury. The flow cytometer showed that the rate of apoptosis was reduced markedly on 7 days at rbFGF group. CONCLUSION: rbFGF can significantly promote the functional repair of injured optic nerve. [

关 键 词：成纤维细胞生长因子 视神经 视网膜神经节细胞 视神经损伤 

分 类 号：R774.6[医药卫生—眼科]