尼可地尔对大鼠脑缺血再灌注损伤的保护作用  

Protective effects of nicorandil on cerebral ischemia-reperfusion injury in rats

作　　者：张轩萍[1] 梁月琴[1] 汤允昭[1] 曹建民 

机构地区：[1]山西医科大学药理教研室,山西太原030001 [2]西山煤电集团公司杜儿坪矿医院,山西太原030053

出　　处：《中国新药与临床杂志》2002年第1期5-8,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的 :观察尼可地尔对大鼠脑缺血再灌注损伤的保护作用。方法 :采用Pulsinelli四动脉结扎法制作大鼠急性前脑缺血再灌注损伤的模型。大鼠随机分成 5组 ,每组 10只 ,剔除死亡和模型不成功后假手术组 6只、再灌注组 9只、尼莫地平 2mg·kg- 1组 6只、尼可地尔 5mg·kg- 1组 7只、尼可地尔10mg·kg- 1组 6只。于缺血前、再灌注前及再灌注过程中各iv给药 1次。结果 :缺血再灌注造成脑组织严重损伤 ,脑水份、脑钙及MDA含量显著增高 ,脑组织LDH含量明显减少 ,脑电图严重抑制 ,锥体细胞坏死明显。尼可地尔能有效地逆转上述变化(P <0 .0 5 )。结论AIM: To study the cerebral protective effects of nicorandil (Nic) on cerebral ischemia reperfusion injury in rats. METHODS: Four vessel occlusion method was used to make acute cerebral ischemia reperfusion model. Rats were divided into 5 groups: sham operation, reperfusion, nimodipine 2 mg·kg -1 , Nic 5 mg·kg -1 and 10 mg·kg -1 groups. Drugs were injected iv to the rats before complete ischemia, before reperfusion and during reperfusion. RESULTS: After 40 min ischemia and 1 h reperfusion in rat brain, brain water content (BWC), brain calcium content (BCC), malondialdehyde (MDA) content were elevated greatly and lactic dehydrogenase (LDH) content was decreased obviously. Nic 5 mg·kg -1 and 10 mg·kg -1 could reduce BWC, decrease BCC, suppress the rise of MDA and increase LDH content. Nic improved the EEG amplitude during reperfusion and reduced the number of dead neurons. CONCLUSION: Nic has great cerebral protective effects against ischemia reperfusion injury.

关 键 词：尼可地尔 钙 再灌注损伤 丙二醛 脑缺血 治疗 

分 类 号：R743.3[医药卫生—神经病学与精神病学]