病毒介导多种基因对胆管癌细胞免疫原性的影响  

Effect of immunogenicity of viral vector-mediated several kinds of genes on cholangiocarcinoma cells

作　　者：王征旭[1] 何振平[2] 胡桂芳[1] 吴祖泽[3] 

机构地区：[1]兰州军区兰州总医院普通外科,兰州730050 [2]第三军医大学附属西南医院全军肝胆外科研究所,西南肝胆外科医院,重庆400038 [3]军事医学科学院放射医学研究所,北京100850

出　　处：《第三军医大学学报》2002年第2期146-148,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 (3 0 0 0 0 0 77)

摘　　要：目的　探讨IFN γ、IL 2或B7 1基因修饰对胆管癌细胞免疫原性的作用。方法　构建携带人IFN γ或IL 2基因的逆转录病毒表达载体 ,和含人B7 1基因的腺病毒载体 ,导入胆管癌细胞系QBC939,运用淋巴细胞杀伤试验、肿瘤细胞在裸鼠体内成瘤性分析、混合淋巴细胞和肿瘤细胞培养 ,研究导入的目的基因对QBC939细胞免疫原性的影响。结果　导入IFN γ基因的QBC939细胞体外能增强胆管癌病人外周血淋巴细胞杀伤亲本瘤细胞的活性 ;导入IL 2基因的QBC939细胞在裸鼠体内的成瘤性降低 ;导入B7 1基因的QBC939细胞能刺激外周血T淋巴细胞的增殖。结论　人IFN γ、IL 2或B7Objective To investigate the change of immunogenicity of cholangiocarcinoma cell line QBC939 modified by human interferon γ, IL 2 or B7 1 genes. Methods The retroviral vector of IL 2 or IFN γ cDNA, and adenoviral vector containing B7 1 cDNA were constructed and transduced into QBC939 cell line. Cytotoxicity lymphocyte assay,tumorigenicity of tumor cell in nude mice, and mixed lymphocyte and tumor cell coculture were employed to study the change of immunogenicity of the cholangiocarcinoma cell line QBC939 transduced with interferon γ, IL 2, or B7 1 gene. Results The cytolytic activity of cholangiocarcinoma patient's peripheral blood lymphocytes against parental cells could be enhanced by in vitro QBC939 after modification with IFN γ gene. The tumorigenicity of QBC939 cell transduced with IL 2 gene could be reduced in nude mice. QBC939 tranduced with B7 1 could stimulate the proliferation of human peripheral blood T lymphocytes. Conclusion The immunogenicity of QBC939 cell lines could be enhanced by human interferon γ, IL 2, or B7 1 cDNA.

关 键 词：胆管癌 干扰素-Γ 白细胞介素-2 B7-1 免疫原性 

分 类 号：R735.8[医药卫生—肿瘤]