地塞米松对小鼠胚胎腭突间充质细胞增殖的影响  

The effect of Dexamethasone on the proliferation of mouse embryonic palatal mesenchymal cells in vitro

作　　者：李伯友 李宏礼[1] 傅豫川[1] 王学斌 

机构地区：[1]武汉大学口腔医学院颌面外科

出　　处：《临床口腔医学杂志》2002年第1期10-12,共3页Journal of Clinical Stomatology

摘　　要：目的　探讨地塞米松对小鼠胚胎腭突间充质 (EPM )细胞增殖的影响。方法　在显微镜下解剖妊娠第 13d的鼠胚腭突 ,用 0 .2 5 %胰蛋白酶进行消化获得游离分散的EPM细胞 ,在DMEM培养基中进行培养 ,并采用反复贴壁法纯化EPM细胞。在培养基中加入 10 6M地塞米松 ,采用AgNOR染色、Feulgen染色及透射电子显微镜观察 ,检测地塞米松对EPM细胞增殖能力的影响。结果　地塞米松处理的EPM细胞银染颗粒数减少 (P <0 .0 1) ,核面积比及DNA合成能力下降 (P <0 .0 1) ,胞浆内线粒体数目减少 ,粗面内质网肿胀。结论　地塞米松对EPM细胞的增殖及生物合成有明显的抑制作用 ,影响腭突的正常发育 。Objective To study the effect of Dexamethasone on the proliferation of mouse EPM cells. Methods The mouse EPM cells were harvested from a female mouse of Day 13 of gestation by microsurgical dissection and digestion with 0.25 % trypsin. The isolated cells were cultured in DMEM medium. Then, 10 6 M Dexamethasone was added to DMEM medium and proliferation capability of the cells was investigated through AgNOR staining, Feulgen staining and transmission electron microscope. Results AgNOR counts of EPM cells were 5.8 ± 0.9 . The integral optical density and nuclear area were 6.2 ± 1.2 , 595±53 μm 2 respectively.When treated with Dexamethasone, AgNOR counts were 1.8 ± 0.4 . The integral optical density and nuclear area were 3.4 ± 1.4 , 282±40 μm 2 . Trasmission electron microscope showed the number of mitochondria decresed and rough endoplasmic reticulum swelled. Conclusion The proliferation of mouse EPM cells is obviously inhibited by Dexamethasone in vitro, which is one of mechanisms of cleft palate.

关 键 词：腭突间充质细胞 胚胎 细胞培养 腭裂 地塞松 细胞增殖 EPM 小鼠 

分 类 号：R782.22[医药卫生—口腔医学]